Publication: Decorin modulates collagen matrix assembly and mineralization.
Issued Date
2009
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Language
eng
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application/pdf
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1628221 bytes
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Mahidol University
Mahidol University
Mahidol University
Bibliographic Citation
Mochida Y, Parisuthiman D, Pornprasertsuk-Damrongsri S, Atsawasuwan P, Sricholpech M, Boskey AL, et al. Decorin modulates collagen matrix assembly and mineralization. Matrix Biol. 2009 Jan; 28(1): 44–52.
Suggested Citation
Suchaya Pornprasertsuk-Damrongsri, Yoshiyuki Mochida, Duenpim Parisuthiman, Phimon Atsawasuwan, Marnisa Sricholpech, Adele L. Boskey, Mitsuo Yamauchi, สุชยา ดำรงค์ศรี Decorin modulates collagen matrix assembly and mineralization.. Mochida Y, Parisuthiman D, Pornprasertsuk-Damrongsri S, Atsawasuwan P, Sricholpech M, Boskey AL, et al. Decorin modulates collagen matrix assembly and mineralization. Matrix Biol. 2009 Jan; 28(1): 44–52.. doi:10.1016/j.matbio.2008.11.003 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/1083
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Title
Decorin modulates collagen matrix assembly and mineralization.
Abstract
Decorin (DCN) is one of the major matrix proteoglycans in bone. To investigate the role of DCN in matrix
mineralization, the expression of DCN in MC3T3-E1 (MC) cell cultures and the phenotypes of MC-derived
clones expressing higher (sense; S-DCN) or lower (antisense; AS-DCN) levels of DCN were characterized. DCN
expression was significantly decreased as the mineralized nodules were formed and expanded in vitro. In
S-DCN clones, in vitro matrix mineralization was inhibited, whereas in AS-DCN clones, mineralization was
accelerated. At the microscopic level, collagen fibers in S-DCN clones were thinner while those of AS-DCN
clones were thicker and lacked directionality compared to the controls. At the ultrastructural level, the collagen
fibrils in S-DCN clones were markedly thinner, whereas those of AS-DCN clones were larger and irregular in
shape. The results from Fourier transform infrared spectroscopy analysis demonstrated that in AS-DCN cultures
the mineral content was greater but the crystallinity of mineral was poorer than that of the controls at early
stage of mineralization. The in vivo transplantation assay demonstrated that no mineralized matrices were
formed in S-DCN transplants, whereas they were readily detected in AS-DCN transplants at 3 weeks of
transplantation. The areas of bone-like matrices in AS-DCN transplants were significantly greater than the
controls at 3 weeks but became comparable at 5 weeks. The bone-like matrices in AS-DCN transplants
exhibited woven bone-like non-lamellar structure while the lamellar bone-like structure was evident in the
control transplants. These results suggest that DCN regulates matrix mineralization by modulating collagen assembly.