Publication: Quantification of the anti-influenza drug zanamivir in plasma using high-throughput HILIC-MS/MS
Issued Date
2011-01-01
Resource Type
ISSN
17576199
17576180
17576180
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2-s2.0-79951925101
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Mahidol University
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SCOPUS
Bibliographic Citation
Bioanalysis. Vol.3, No.2 (2011), 157-165
Suggested Citation
Niklas Lindegardh, Warunee Hanpithakpong, Benjamas Kamanikom, Jeremy Farrar, Tran Tinh Hien, Pratap Singhasivanon, Nicholas J. White, Nicholas P J Day Quantification of the anti-influenza drug zanamivir in plasma using high-throughput HILIC-MS/MS. Bioanalysis. Vol.3, No.2 (2011), 157-165. doi:10.4155/bio.10.189 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11635
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Title
Quantification of the anti-influenza drug zanamivir in plasma using high-throughput HILIC-MS/MS
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Abstract
Background: Parenteral zanamivir is a promising drug for the treatment of severe influenza. However, quantification of this polar drug in biological matrices has traditionally been difficult and the methods developed have been relatively insensitive. Results: A high-throughput bioanalytical method for the analysis of zanamivir in human plasma using SPE in the 96-well plate format and LC coupled to positive MS/MS has been developed and validated according to US FDA guidelines. The method uses 50 Âμ l of plasma and covers a large working range from 1-50, 000 ng/ml with a LOD of 0.50 ng/ml. Conclusion: This new LC-MS/MS assay is more sensitive than previous methods despite using a small plasma volume sample. It is particularly suitable for clinical studies on both parenteral and inhaled zanamivir. © 2011 Future Science Ltd.