Publication: Effects of paraoxon on neuronal and lymphocytic cholinergic systems
Issued Date
2011-01-01
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ISSN
13826689
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2-s2.0-78650204708
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Mahidol University
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SCOPUS
Bibliographic Citation
Environmental Toxicology and Pharmacology. Vol.31, No.1 (2011), 119-128
Suggested Citation
Tanvisith Charoenying, Tawit Suriyo, Apinya Thiantanawat, Sansanee C. Chaiyaroj, Preeda Parkpian, Jutamaad Satayavivad Effects of paraoxon on neuronal and lymphocytic cholinergic systems. Environmental Toxicology and Pharmacology. Vol.31, No.1 (2011), 119-128. doi:10.1016/j.etap.2010.09.012 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11935
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Title
Effects of paraoxon on neuronal and lymphocytic cholinergic systems
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Abstract
The cholinergic system in lymphocytes is hypothesized to be a key target for neurotoxic organophosphates (OPs). The present study determined the comparative effects of paraoxon, the active metabolite of OP-parathion, which is detected in the human neuroblastoma line, SH-SY5Y, and leukemic T-lymphocytes, MOLT-3, in vitro. Paraoxon induced cytotoxic effects in a dose- and time-dependent manner in both cells. Further, the paraoxon-induced modulatory effects were comparable despite different cell types, including over-expression of N-terminus acetylcholinesterase (N-AChE) protein, a marker of apoptosis, down-regulations of mRNA encoding M1, M2, and M3 muscarinic acetylcholine receptors (mAChRs), and induction in expression of c-Fos gene, an indication of certain mAChR subtype(s) activation. Furthermore, the non-selective cholinergic antagonist atropine partially attenuated the paraoxon-induced N-AChE and c-Fos activations in both types of cells. These results provide initial and additional information that OPs may similarly induce neuro- and immuno-toxic effects through mAChRs activation, and they underline the potential of using lymphocytes for assessing OPs-induced neurotoxicity. © 2010.