Publication: Cryopreservation of immature buffalo oocytes: Effects of cytochalasin B pretreatment on the efficiency of cryotop and solid surface vitrification methods
Issued Date
2012-09-01
Resource Type
ISSN
17400929
13443941
13443941
Other identifier(s)
2-s2.0-84865730594
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Animal Science Journal. Vol.83, No.9 (2012), 630-638
Suggested Citation
Yuanyuan Liang, Damrongrak Rakwongrit, Tatsanee Phermthai, Tamas Somfai, Takashi Nagai, Rangsun Parnpai Cryopreservation of immature buffalo oocytes: Effects of cytochalasin B pretreatment on the efficiency of cryotop and solid surface vitrification methods. Animal Science Journal. Vol.83, No.9 (2012), 630-638. doi:10.1111/j.1740-0929.2012.01013.x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13404
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Cryopreservation of immature buffalo oocytes: Effects of cytochalasin B pretreatment on the efficiency of cryotop and solid surface vitrification methods
Abstract
The aim of the present study was to compare the efficiency of the solid surface (SSV), cryotop (CT) vitrification methods and cytochalasin B (CB) pretreatment for cryopreservation of immature buffalo oocytes. Cumulus-oocyte complexes (COCs) were placed for 1min in TCM199 containing 10% dimethylsulfoxide (DMSO), 10% ethylene glycol (EG), and 20% fetal bovine serum, and then transferred for 30s to base medium containing 20% DMSO, 20% EG and 0.5mol/L sucrose. CB pretreated ((+)CB) or non-pretreated ((-)CB) COCs were vitrified either by SSV or CT. Surviving vitrified COCs were selected for in vitro maturation (IVM) and in vitro fertilization (IVF). The rate of viable oocytes after vitrification in CT groups (82%) was significantly lower (P < 0.05) than that in a fresh control group (100%), but significantly higher (P < 0.05) than those in SSV groups (71-72%). Among vitrified groups, the highest maturation rate was obtained in the CT (-)CB group (32%). After IVF, the cleavage and blastocyst formation rates were similar among vitrified groups but significantly lower than those of the control group. In conclusion, a higher survival rate of oocytes after vitrification and IVM was obtained in the CT group compared with that in the SSV group, indicating the superiority of the CT method. Pretreatment with CB did not increase the viability, maturation or embryo development of vitrified oocytes. © 2012 The Authors. Animal Science Journal © 2012 Japanese Society of Animal Science.