Publication: Interaction of dengue virus nonstructural protein 5 with Daxx modulates RANTES production
Issued Date
2012-06-29
Resource Type
ISSN
10902104
0006291X
0006291X
Other identifier(s)
2-s2.0-84862861520
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.423, No.2 (2012), 398-403
Suggested Citation
Sasiprapa Khunchai, Mutita Junking, Aroonroong Suttitheptumrong, Umpa Yasamut, Nunghathai Sawasdee, Janjuree Netsawang, Atthapan Morchang, Prapaipit Chaowalit, Sansanee Noisakran, Pa thai Yenchitsomanus, Thawornchai Limjindaporn Interaction of dengue virus nonstructural protein 5 with Daxx modulates RANTES production. Biochemical and Biophysical Research Communications. Vol.423, No.2 (2012), 398-403. doi:10.1016/j.bbrc.2012.05.137 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13691
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Title
Interaction of dengue virus nonstructural protein 5 with Daxx modulates RANTES production
Abstract
Dengue fever (DF), dengue hemorrhagic fever (DHF), and dengue shock syndrome (DSS), caused by dengue virus (DENV) infection, are important public health problems in the tropical and subtropical regions. Abnormal hemostasis and plasma leakage are the main patho-physio logical changes in DHF/DSS. A remarkably increased production of cytokines, the so called 'cytokine storm', is observed in the patients with DHF/DSS. A complex interaction between DENV proteins and the host immune response contributes to cytokine production. However, the molecular mechanism(s) by which DENV nonstructural protein 5 (NS5) mediates these responses has not been fully elucidated. In the present study, yeast two-hybrid assay was performed to identify host proteins interacting with DENV NS5 and a death-domain-associate protein (Daxx) was identified. The in vivo relevance of this interaction was suggested by co-immunoprecipitation and nuclear co-localization of these two proteins in HEK293 cells expressing DENV NS5. HEK293 cells expressing DENV NS5-K/A, which were mutated at the nuclear localization sequences (NLS), were created to assess its functional roles in nuclear translocation, Daxx interaction, and cytokine production. In the absence of NLS, DENV NS5 could neither translocate into the nucleus nor interact with Daxx to increase the DHF-associated cytokine, RANTES (CCL5) production. This work demonstrates the interaction between DENV NS5 and Daxx and the role of the interaction on the modulation of RANTES production. © 2012 Elsevier Inc.