Publication: Investigations on the role of a lysozyme from the malaria vector Anopheles dirus during malaria parasite development
Issued Date
2012-01-01
Resource Type
ISSN
0145305X
Other identifier(s)
2-s2.0-80054928001
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Mahidol University
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SCOPUS
Bibliographic Citation
Developmental and Comparative Immunology. Vol.36, No.1 (2012), 104-111
Suggested Citation
Parichat Lapcharoen, Narumon Komalamisra, Yupha Rongsriyam, Voranuch Wangsuphachart, Paron Dekumyoy, Jetsumon Prachumsri, Mayur K. Kajla, Susan M. Paskewitz Investigations on the role of a lysozyme from the malaria vector Anopheles dirus during malaria parasite development. Developmental and Comparative Immunology. Vol.36, No.1 (2012), 104-111. doi:10.1016/j.dci.2011.06.010 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13857
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Title
Investigations on the role of a lysozyme from the malaria vector Anopheles dirus during malaria parasite development
Abstract
A cDNA encoding a lysozyme was obtained by rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR) from females of the malaria vector Anopheles dirus A (Diptera: Culicidae). The 623. bp lysozyme (AdLys c-1) cDNA encodes the 120 amino acid mature protein with a predicted molecular mass of 13.4. kDa and theoretical pI of 8.45. Six cysteine residues and a potential calcium binding motif that are present i n AdLys c-1 are highly conserved relative to those of c-type lysozymes found in other insects. RT-PCR analysis of the AdLys c-1 transcript revealed its presence at high levels in the salivary glands both in larval and adult stages and in the larval caecum. dsRNA mediated gene knockdown experiments were conducted to examine the potential role of this lysozyme during Plasmodium berghei infection. Silencing of AdLys c-1 resulted in a significant reduction in the number of oocysts as compared to control dsGFP injected mosquitoes. © 2011 Elsevier Ltd.