Publication: Identification of prohibitin as a Chikungunya virus receptor protein
Issued Date
2012-11-01
Resource Type
ISSN
10969071
01466615
01466615
Other identifier(s)
2-s2.0-84866485831
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Medical Virology. Vol.84, No.11 (2012), 1757-1770
Suggested Citation
Phitchayapak Wintachai, Nitwara Wikan, Atichat Kuadkitkan, Thitigun Jaimipuk, Sukathida Ubol, Rojjanaporn Pulmanausahakul, Prasert Auewarakul, Watchara Kasinrerk, Wen Yu Weng, Mingkwan Panyasrivanit, Atchara Paemanee, Suthathip Kittisenachai, Sittiruk Roytrakul, Duncan R. Smith Identification of prohibitin as a Chikungunya virus receptor protein. Journal of Medical Virology. Vol.84, No.11 (2012), 1757-1770. doi:10.1002/jmv.23403 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/14246
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Identification of prohibitin as a Chikungunya virus receptor protein
Abstract
Chikungunya virus (CHIKV) has recently re-emerged causing millions of infections in countries around the Indian Ocean. While CHIKV has a broad host cell range and productively infects a number of different cell types, macrophages have been identified as a potential viral reservoir serving to increase the duration of symptoms. To date no CHIKV interacting protein has been characterized and this study sought to identify CHIKV binding proteins expressed on target cell membranes. Two-dimensional virus overlay identified prohibitin (PHB) as a microglial cell expressed CHIKV binding protein. Co-localization, co-immunoprecipitation as well as antibody and siRNA mediated infection inhibition studies all confirmed a role for PHB in mediating internalization of CHIKV into microglial cells. PHB is the first identified CHIKV receptor protein, and this study is evidence that PHB may play a role in the internalization of multiple viruses. © 2012 Wiley Periodicals, Inc.