Publication: Genotyping of HPA-1 to -7 and -15 in the Thai population using multiplex PCR
Issued Date
2012-08-01
Resource Type
ISSN
13653148
09587578
09587578
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2-s2.0-84864039672
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Mahidol University
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SCOPUS
Bibliographic Citation
Transfusion Medicine. Vol.22, No.4 (2012), 272-276
Suggested Citation
M. Kengkate, P. Butthep, P. Kupatawintu, S. Kanunthong, W. Chantratita, O. Nathalang Genotyping of HPA-1 to -7 and -15 in the Thai population using multiplex PCR. Transfusion Medicine. Vol.22, No.4 (2012), 272-276. doi:10.1111/j.1365-3148.2012.01153.x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/14707
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Title
Genotyping of HPA-1 to -7 and -15 in the Thai population using multiplex PCR
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Abstract
Background: Different polymerase chain reaction (PCR) techniques for human platelet antigens (HPA) genotyping have been implemented, in order to diagnose the clinical syndromes of patients with thrombocytopaenia and provide effective HPA-matched platelet donors. Objectives: The aim of this study is to develop an in-house multiplex PCR for HPA-1 to -7 and -15 genotyping in the Thai population. Methods: One hundred DNA samples of known HPA genotyping by the PCR with sequence-specific primers (PCR-SSP), as previously described, were tested with the multiplex PCR. Additionally, 300 DNA samples of group O donors were tested for HPA-1 to -7 and -15 genotyping using multiplex PCR. Results: The comparison of HPA-1 to -7 and -15 genotype results between multiplex PCR and PCR-SSP technique was in 100% concordance. Interestingly, HPA-2b2b genotype was found in two samples; however, other low-incidence genotypes such as HPA-1b1b, HPA-5b5b, HPA-6b6b and HPA-7b7b were not found in this study. Moreover, 30 samples were randomly tested twice for HPA genotyping using the multiplex PCR and demonstrated reproducible results. Conclusions: This study shows that the in-house multiplex PCR is simple, cost-effective and suitable for HPA genotyping for routine laboratories in other developing countries. Nevertheless, a large-scale evaluation of this technique through multicentre analysis is suggested. © 2012 The Authors. Transfusion Medicine © 2012 British Blood Transfusion Society.