Publication:
Detection of β-thalassemia and hemoglobin E genes in Thai by a DNA amplification technique

Issued Date

1989-07-01

Resource Type

Article

ISSN

14321203
03406717

DOI

10.1007/BF00274004

Other identifier(s)

2-s2.0-0024340685

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Human Genetics. Vol.82, No.4 (1989), 389-390

Suggested Citation

Pranee Winichagoon, Jiraporn Kownkon, Pathai Yenchitsomanus, Varaporn Thonglairoam, Nopadol Siritanaratkul, Suthat Fucharoen Detection of β-thalassemia and hemoglobin E genes in Thai by a DNA amplification technique. Human Genetics. Vol.82, No.4 (1989), 389-390. doi:10.1007/BF00274004 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/15709

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Title

Detection of β-thalassemia and hemoglobin E genes in Thai by a DNA amplification technique

Author(s)

Pranee Winichagoon
 Jiraporn Kownkon
 Pathai Yenchitsomanus
 Varaporn Thonglairoam
 Nopadol Siritanaratkul
 Suthat Fucharoen

Other Contributor(s)

Mahidol University

Abstract

Enzymatic DNA amplification and polyacrylamide gel electrophoresis, which demonstrate different sizes of DNA fragments, were used to detect the common mutations causing β-thalassemia and hemoglobin (Hb) E in Thai people. The 4-bp deletion at codons 41 and 42 can be detected directly by polyacrylamide gel electrophoresis and ethidium bromide staining. Whereas the nonsense mutations at codon 17 (AAG →TAG) and Hb E (GAG→AAG at codon 26) were detected after digestion of the amplified DNA with the enzymes MaeI and MnlI, respectively. © 1989 Springer-Verlag.

Keyword(s)

Biochemistry, Genetics and Molecular Biology
 Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/15709

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Scopus 1969-1990