Publication: Molecular analysis of unknown β-globin gene mutations using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique and its application in Thai families with β-thalassemias and β-globin variants
Issued Date
2005-11-01
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ISSN
00099120
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2-s2.0-27744574280
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Mahidol University
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SCOPUS
Bibliographic Citation
Clinical Biochemistry. Vol.38, No.11 (2005), 987-996
Suggested Citation
Worrawut Chinchang, Vip Viprakasit, Parichat Pung-Amritt, Voravarn S. Tanphaichitr, Pa Thai Yenchitsomanus Molecular analysis of unknown β-globin gene mutations using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique and its application in Thai families with β-thalassemias and β-globin variants. Clinical Biochemistry. Vol.38, No.11 (2005), 987-996. doi:10.1016/j.clinbiochem.2005.07.013 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16275
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Title
Molecular analysis of unknown β-globin gene mutations using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique and its application in Thai families with β-thalassemias and β-globin variants
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Abstract
Objectives: Approximately 40 β-globin gene mutations have been identified in Thailand. The detection of these mutations is currently performed by the reverse dot blot (RDB) hybridization technique, which could detect only known mutations. We describe here the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) assay for detecting unknown mutations of the β-globin genes. Design and methods: Six PCR fragments covering the promoter, entire coding region, and intervening sequences were amplified before separation by the SSCP technique. Fifteen known mutations and two polymorphisms were analyzed by this technique in various gel mixtures and temperatures to compare their mobility shift patterns. Results: The clear patterns of mobility shift were demonstrated when a 10% polyacrylamide gel with 5% glycerol was used. The sensitivity was found to be 100% when electrophoreses were performed at both room temperature and 6°C. This technique was then applied to screen β-globin gene mutations in Thai families with similar profiles of abnormal hemoglobins. The distinct patterns of mobility shifts were observed in which further sequencing analysis revealed an AC insertion at codon 146, causing hemoglobin Tak. Conclusion: The PCR-SSCP technique might be a useful molecular technique to minimize the requirement of direct genomic sequencing to identify β-globin gene mutations and could be applied in several developing countries where resources are limited but genetic hemoglobin disorders are highly prevalent. © 2005 The Canadian Society of Clinical Chemists. All rights reserved.