Publication: Short report: Detection of Orientia tsutsugamushi in clinical samples by quantitative real-time polymerase chain reaction
Issued Date
2005-05-01
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ISSN
00029637
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2-s2.0-20544466465
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.72, No.5 (2005), 640-641
Suggested Citation
Tasawan Singhsilarak, Wattana Leowattana, Sornchailoo Areesuwan, Varee Wongchotigul, Ju Jiang, Allen L. Richards, George Watt Short report: Detection of Orientia tsutsugamushi in clinical samples by quantitative real-time polymerase chain reaction. American Journal of Tropical Medicine and Hygiene. Vol.72, No.5 (2005), 640-641. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16589
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Title
Short report: Detection of Orientia tsutsugamushi in clinical samples by quantitative real-time polymerase chain reaction
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Abstract
Orientia tsutsugamushi infection causes scrub typhus, a common zoonosis of rural Asia. Orientia tsutsugamushi was recently detected by a real-time quantitative polymerase chain reaction (qPCR) assay in animal specimens. We evaluated the same qPCR assay in specimens obtained from patients with serologically proven scrub typhus infections. The 47-kDa qPCR assay was more sensitive than was mouse inoculation; it was reactive in whole blood specimens from all 10 isolate-positive patients and in 7 of 17 isolate-negative individuals (P = 0.003, Fisher's two-tailed exact test). As few as 1,076 O. tsutsugamushi copies/μL were detected in whole blood. Four of 7 sera from isolate-proven scrub typhus infections were also reactive by qPCR. The assay was unreactive in all 12 individuals without scrub typhus infection. This is the first demonstration of a sensitive and specific real-time qPCR assay for human scrub typhus infection. Copyright © 2005 by The American Society of Tropical Medicine and Hygiene.