Publication: Molecular characterization of the complete genome of human influenza H5N1 virus isolates from Thailand
Issued Date
2005-02-01
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ISSN
00221317
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2-s2.0-13644270916
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of General Virology. Vol.86, No.2 (2005), 423-433
Suggested Citation
Pilaipan Puthavathana, Prasert Auewarakul, Pakapak Chor Charoenying, Kantima Sangsiriwut, Phisanu Pooruk, Kobporn Boonnak, Raweewan Khanyok, Pranee Thawachsupa, Rungrueng Kijphati, Pathom Sawanpanyalert Molecular characterization of the complete genome of human influenza H5N1 virus isolates from Thailand. Journal of General Virology. Vol.86, No.2 (2005), 423-433. doi:10.1099/vir.0.80368-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16608
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Title
Molecular characterization of the complete genome of human influenza H5N1 virus isolates from Thailand
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Abstract
The complete genomes of three human H5N1 influenza isolates were characterized, together with the haemagglutinin (HA) and neuraminidase (NA) genes from two additional human isolates and one chicken isolate. These six influenza isolates were obtained from four different provinces of Thailand during the avian influenza outbreak in Asia from late 2003 to May 2004. All six Thailand isolates contained multiple basic amino acids at the cleavage site in the HA gene. Amino acid residues at the receptor-binding site of the five human viruses were similar to those of the chicken virus and other H5N1 viruses from Hong Kong. The presence of amantadine resistance in the Thailand viruses isolated during this outbreak was suggested by a fixed mutation in M2 and confirmed by a phenotypic assay. All genomic segments of the Thailand viruses clustered with the recently described genotype Z. The Thailand viruses contained more avian-specific residues than the 1997 Hong Kong H5N1 viruses, suggesting that the virus may have adapted to allow a more efficient spread in avian species. © 2005 SGM.