Publication: Susceptibility of Mansonia uniformis to Brugia malayi microfilariae from infected domestic cat
Issued Date
2005-03-01
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ISSN
01251562
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2-s2.0-20444489971
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.36, No.2 (2005), 434-441
Suggested Citation
Usa Lek-Uthai, Wanachai Tomoen Susceptibility of Mansonia uniformis to Brugia malayi microfilariae from infected domestic cat. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.36, No.2 (2005), 434-441. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17041
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Title
Susceptibility of Mansonia uniformis to Brugia malayi microfilariae from infected domestic cat
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Abstract
Microfilariae of Brugia malayi is transmitted to man and other susceptible hosts via mosquito. The transmission of B. malayi from cat to man by Ma. uniformis bite has never been reported. The Ma. uniformis mosquito is the normal vector for Wuchereria bancrofti but has never been reported as a vector for B. malayi, or a susceptible host for the growth and development of the microfilariae of B. malayi. The purpose of this study was to examine the development of B. malayi in Mansonia uniformis after feeding on the blood of an infected cat in the laboratory. The B. malayi infected cat was identified using PCR with the primers Bm- 1/Bm-2 on DNA (at 10 ng/50 μl) extracted from the WBC of the cat. W. bancrofti was employed as a negative control. The sensitivity of the B. malayi DNA detection by PCR was 0.0001 ng. Adult Ma. uniformis mosquitos at the ages of 5, 10, and 15 days, 100 mosquitos in each group, were fed on the infected cat blood. Recovery of third stage microfilariae was found to be the highest in the 5-day old mosquito group (48%), followed by the 10- and 15-day old mosquito groups (32% and 18 %, respectively). The mean number of B. malayi microfilariae found in thorax, head, and abdomen of the mosquitos were composed. The 5-day old (40.3%) and 10-day old (41.9%) mosquitos were significantly more susceptible to microfilariae than the 15-day old mosquitos (17.8%) (p-values using the Scheffe method: 0.027 and 0.039, respectively). There was no significant difference in the mean number of microfilariae in the thorax (p=0.482) by age, but the mean numbers of microfilariae in the heads, and abdomens were significantly different by age between the 5- and 10-, and the 15-day old mosquitos (p<0.001 and p=0.004, respectively).