Publication: Detection of HIV-1 Window Period Infection in Blood Donors Using Borderline Anti-HIV Results, HIV-1 Proviral DNA PCR, and HIV-1 Antigen Test
Issued Date
1997-09-01
Resource Type
ISSN
01252208
Other identifier(s)
2-s2.0-0031225835
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol.80, No.SUPPL. 1 (1997)
Suggested Citation
Wachanan Wongsena, Mongkol Kunakorn, Srivilai Tanprasert, Bencha Petchclai Detection of HIV-1 Window Period Infection in Blood Donors Using Borderline Anti-HIV Results, HIV-1 Proviral DNA PCR, and HIV-1 Antigen Test. Journal of the Medical Association of Thailand. Vol.80, No.SUPPL. 1 (1997). Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18095
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Detection of HIV-1 Window Period Infection in Blood Donors Using Borderline Anti-HIV Results, HIV-1 Proviral DNA PCR, and HIV-1 Antigen Test
Other Contributor(s)
Abstract
Prevention of transmission of HTV-1 via blood transfusion has been carried out by the National Blood Center by screening donated blood with anti-HIV and HIV antigen tests. To increase the safety measure, detection of proviral DNA by PCR has been proposed; however, it was impractical to test all samples by PCR. From August 1994 to September 1995, there were 296,169 blood donors with 0.32 per cent prevalence of anti-HIV positive. From these donors, 153 samples of which the anti-HIV enzyme immunoassay optical density (OD) between cutoff and 80 per cent of cutoff value (borderline results) were selected for PCR testing. One out of 153 borderline cases showed positive by PCR test for HTV-1 proviral DNA. However, this case was also positive by HIV antigen test. Therefore, most of the samples with borderline anti-HIV results were true negative for HIV infection. On the other hand, there were 8 HIV antigen positive samples which had anti-HIV OD below the borderline value determined in this study. This finding confirmed the necessity of using both the anti-HIV and HIV antigen tests for screening of donated blood.