Publication: Immunogenicity and protective role of three formulations of oral cholera vaccine
Issued Date
1998-01-01
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ISSN
0264410X
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2-s2.0-0031986143
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Mahidol University
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SCOPUS
Bibliographic Citation
Vaccine. Vol.16, No.2-3 (1998), 201-207
Suggested Citation
Thareerat Kalambaheti, Urai Chaisri, Potjanee Srimanote, Emsri Pongponratn, Wanpen Chaicumpa Immunogenicity and protective role of three formulations of oral cholera vaccine. Vaccine. Vol.16, No.2-3 (1998), 201-207. doi:10.1016/S0264-410X(97)88330-2 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18316
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Title
Immunogenicity and protective role of three formulations of oral cholera vaccine
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Abstract
Three formulations of oral cholera vaccine were compared with respect to their immunogenicity and protective ability in a rat ileal loop model. Eight-week-old Wistar rats were divided into five groups. The first group received orally vaccine A consisting of liposome-associated V. cholerae lipopolysaccharide, fimbriae and procholeragenoid, whereas the rats of groups 2 and 3 received orally vaccines B and C consisting of heat-killed fimbriated and non-fimbriated whole cell V. cholerae, respectively. Rats of groups 4 and 5 were controls that received orally liposomes alone and normal saline solution, respectively. It was found that vaccine A elicited stronger immune responses to all three V. cholerae antigens. The antibody responses were detected in both serum and intestinal lavage samples. Vaccine B elicited only modest serum and intestinal responses to V. cholerae fimbriae (anti-F). No detectable immune response was found in rats of group 3 immunized with vaccine C. Rats immunized with vaccines A and B had a similar order of magnitude of numbers of vibrios adhered to their intestinal mucosa. These numbers were less than those associated with the intestinal tissues of control rats of groups 4 and 5 by about two orders of magnitude. Although without any detectable immune response, rats of group 3 that were immunized with vaccine C showed some reduction in numbers of vibrios associated with their intestinal mucosa. The numbers of vibrios recovered from the intestinal segments of rats of all treatment groups were in the order group 1 = 2 < 4 = 5. Electron micrography also revealed patches of vibrio colonization on the mucosa of rats of groups 3, 4 and 5. These features were not found in the groups vaccinated with vaccines A and B. The inhibition of vibrio colonization afforded by the vaccines was biotype- and serotype-non-specific. The results suggest that the heat-killed whole cell fimbriated V. cholerae may be an alternative vaccine preparation to the liposome-associated refined antigen vaccine at a lower cost.