Publication: The core and accessory genomes of Burkholderia pseudomallei: Implications for human melioidosis
Issued Date
2008-10-01
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ISSN
15537374
15537366
15537366
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2-s2.0-55449099635
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Mahidol University
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SCOPUS
Bibliographic Citation
PLoS Pathogens. Vol.4, No.10 (2008)
Suggested Citation
Hoon Sim Siew, Yiting Yu, Ho Lin Chi, R. Krishna M. Karuturi, Vanaporn Wuthiekanun, Apichai Tuanyok, Hoon Chua Hui, Catherine Ong, Sivalingam Suppiah Paramalingam, Gladys Tan, Lynn Tang, Gary Lau, Eong Ooi Eng, Donald Woods, Edward Feil, Sharon J. Peacock, Patrick Tan The core and accessory genomes of Burkholderia pseudomallei: Implications for human melioidosis. PLoS Pathogens. Vol.4, No.10 (2008). doi:10.1371/journal.ppat.1000178 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18854
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Title
The core and accessory genomes of Burkholderia pseudomallei: Implications for human melioidosis
Abstract
Natural isolates of Burkholderia pseudomallei (Bp), the causative agent of melioidosis, can exhibit significant ecological flexibility that is likely reflective of a dynamic genome. Using whole-genome Bp microarrays, we examined patterns of gene presence and absence across 94 South East Asian strains isolated from a variety of clinical, environmental, or animal sources. 86% of the Bp K96243 reference genome was common to all the strains representing the Bp "core genome", comprising genes largely involved in essential functions (eg amino acid metabolism, protein translation). In contrast, 14% of the K96243 genome was variably present across the isolates. This Bp accessory genome encompassed multiple genomic islands (GIs), paralogous genes, and insertions/deletions, including three distinct lipopolysaccharide (LPS)-related gene clusters. Strikingly, strains recovered from cases of human melioidosis clustered on a tree based on accessory gene content, and were significantly more likely to harbor certain GIs compared to animal and environmental isolates. Consistent with the inference that the GIs may contribute to pathogenesis, experimental mutation of BPSS2053, a GI gene, reduced microbial adherence to human epithelial cells. Our results suggest that the Bp accessory genome is likely to play an important role in microbial adaptation and virulence. © 2008 Sim et al.