Publication: Rapid genotyping of loci involved in antifolate drug resistance in Plasmodium falciparum by primer extension
Issued Date
2002-06-20
Resource Type
ISSN
00207519
Other identifier(s)
2-s2.0-0036275783
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal for Parasitology. Vol.32, No.7 (2002), 852-858
Suggested Citation
Shalini Nair, Alan Brockman, Lucy Paiphun, François Nosten, Tim J C Anderson Rapid genotyping of loci involved in antifolate drug resistance in Plasmodium falciparum by primer extension. International Journal for Parasitology. Vol.32, No.7 (2002), 852-858. doi:10.1016/S0020-7519(02)00033-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20205
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Title
Rapid genotyping of loci involved in antifolate drug resistance in Plasmodium falciparum by primer extension
Abstract
Current methods used to genotype point mutations in Plasmodium falciparum genes involved in resistance to antifolate drugs include restriction digestion of PCR products, allele-specific amplification or sequencing. Here we demonstrate that known point mutations in dihydrofolate reductase and dihydropteroate synthase can be scored quickly and accurately by single-nucleotide primer extension and detection of florescent products on a capillary sequencer. We use this method to genotype parasites in natural infections from the Thai-Myanmar border. This approach could greatly simplify large-scale screening of resistance mutations of the type required for evaluating and updating antimalarial drug treatment policies. The method can be easily adapted to other P. falciparum genes and will greatly simplify scoring of point mutations in this and other parasitic organisms. © 2002 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.