Publication:
Complete genomes of two clinical Staphylococcus aureus strains: Evidence for the evolution of virulence and drug resistance

Issued Date

2004-06-29

Resource Type

Article

ISSN

00278424

DOI

10.1073/pnas.0402521101

Other identifier(s)

2-s2.0-3042718925

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Proceedings of the National Academy of Sciences of the United States of America. Vol.101, No.26 (2004), 9786-9791

Suggested Citation

Matthew T.G. Holden, Edward J. Feil, Jodi A. Lindsay, Sharon J. Peacock, Nicholas P.J. Day, Mark C. Enright, Tim J. Foster, Catrin E. Moore, Laurence Hurst, Rebecca Atkin, Andrew Barron, Nathalie Bason, Stephen D. Bentley, Carol Chillingworth, Tracey Chillingworth, Carol Churcher, Louise Clark, Craig Corton, Ann Cronin, Jon Doggett, Linda Dowd, Theresa Feltwell, Zahra Hance, Barbara Harris, Heidi Hauser, Simon Holroyd, Kay Jagels, Keith D. James, Nicola Lennard, Alexandra Line, Rebecca Mayes, Sharon Moule, Karen Mungall, Douglas Ormond, Michael A. Quail, Ester Rabbinowitsch, Kim Rutherford, Mandy Sanders, Sarah Sharp, Mark Simmonds, Kim Stevens, Sally Whitehead, Bart G. Barrell, Brian G. Spratt, Julian Parkhill Complete genomes of two clinical Staphylococcus aureus strains: Evidence for the evolution of virulence and drug resistance. Proceedings of the National Academy of Sciences of the United States of America. Vol.101, No.26 (2004), 9786-9791. doi:10.1073/pnas.0402521101 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21832

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Title

Complete genomes of two clinical Staphylococcus aureus strains: Evidence for the evolution of virulence and drug resistance

Author(s)

Matthew T.G. Holden
 Edward J. Feil
 Jodi A. Lindsay
 Sharon J. Peacock
 Nicholas P.J. Day
 Mark C. Enright
 Tim J. Foster
 Catrin E. Moore
 Laurence Hurst
 Rebecca Atkin
 Andrew Barron
 Nathalie Bason
 Stephen D. Bentley
 Carol Chillingworth
 Tracey Chillingworth
 Carol Churcher
 Louise Clark
 Craig Corton
 Ann Cronin
 Jon Doggett
 Linda Dowd
 Theresa Feltwell
 Zahra Hance
 Barbara Harris
 Heidi Hauser
 Simon Holroyd
 Kay Jagels
 Keith D. James
 Nicola Lennard
 Alexandra Line
 Rebecca Mayes
 Sharon Moule
 Karen Mungall
 Douglas Ormond
 Michael A. Quail
 Ester Rabbinowitsch
 Kim Rutherford
 Mandy Sanders
 Sarah Sharp
 Mark Simmonds
 Kim Stevens
 Sally Whitehead
 Bart G. Barrell
 Brian G. Spratt
 Julian Parkhill

Other Contributor(s)

Wellcome Trust Sanger Institute
 University of Bath
 St George's University of London
 Nuffield Department of Clinical Medicine
 Trinity College Dublin
 St Mary's Hospital London
 Mahidol University

Abstract

Staphylococcus aureus is an important nosocomial and community-acquired pathogen. Its genetic plasticity has facilitated the evolution of many virulent and drug-resistant strains, presenting a major and constantly changing clinical challenge. We sequenced the ≈2.8-Mbp genomes of two disease-causing S. aureus strains isolated from distinct clinical settings: a recent hospital-acquired representative of the epidemic methicillin-resistant S. aureus EMRSA-16 clone (MRSA252), a clinically important and globally prevalent lineage; and a representative of an invasive community-acquired methicillin-susceptible S. aureus clone (MSSA476). A comparative-genomics approach was used to explore the mechanisms of evolution of clinically important S. aureus genomes and to identify regions affecting virulence and drug resistance. The genome sequences of MRSA252 and MSSA476 have a well conserved core region but differ markedly in their accessory genetic elements. MRSA252 is the most genetically diverse S. aureus strain sequenced to date: ≈6% of the genome is novel compared with other published genomes, and it contains several unique genetic elements. MSSA476 is methicillin-susceptible, but it contains a novel Staphylococcal chromosomal cassette (SCC) mec-like element (designated SCC476), which is integrated at the same site on the chromosome as SCCmec elements in MRSA strains but encodes a putative fusidic acid resistance protein. The crucial role that accessory elements play in the rapid evolution of S. aureus is clearly illustrated by comparing the MSSA476 genome with that of an extremely closely related MRSA community-acquired strain; the differential distribution of large mobile elements carrying virulence and drug-resistance determinants may be responsible for the clinically important phenotypic differences in these strains.

Keyword(s)

Multidisciplinary

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/21832

Collections

Scopus 2001-2005