Publication: Effects of n<sup>6</sup>, 2′-O-dibutyryl cyclic adenosine monophosphate and the leukemia inhibitory factor on embryo development of the giant freshwater prawn, Macrobrachium rosenbergii
Issued Date
2006-01-01
Resource Type
ISSN
07924259
Other identifier(s)
2-s2.0-33747594977
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Invertebrate Reproduction and Development. Vol.49, No.1-2 (2006), 71-77
Suggested Citation
Praneet Damrongphol, Ketkaew Suwannarong Effects of n<sup>6</sup>, 2′-O-dibutyryl cyclic adenosine monophosphate and the leukemia inhibitory factor on embryo development of the giant freshwater prawn, Macrobrachium rosenbergii. Invertebrate Reproduction and Development. Vol.49, No.1-2 (2006), 71-77. doi:10.1080/07924259.2006.9652195 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/22921
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Effects of n<sup>6</sup>, 2′-O-dibutyryl cyclic adenosine monophosphate and the leukemia inhibitory factor on embryo development of the giant freshwater prawn, Macrobrachium rosenbergii
Author(s)
Abstract
The effects of N6, 2′-O-dibutyryl cyclic adenosine monophosphate (dbcAMP) and the leukemia inhibitory factor (LIF) on embryo development of the giant freshwater prawn, Macrobrachium rosenbergii, were studied. Embryos 1.5 days old were cultured in vitro in 15% artificial seawater supplemented with 0.05 mM, 0.1 mM or 0.5 mM dbcAMP, or with 10 ng/ml, 20 ng/ml or 50 ng/ml LIF for 2 days. No differences in the morphology or growth of the embryos between the supplemented and the control groups were observed. The developmental rates as revealed by the eye formation rates were not altered with either supplement. The survival rates of the embryos declined during the course of development; and, again, the rates were not altered when supplemented with dbcAMP or LIF. Although there were no significant changes in the eye formation rate in embryos supplemented with 0.05 mM dbcAMP, an increase in the hatching rate was found. In contrast, supplementation with LIF did not improve the hatching rates. Germ cell development seemed to be most affected. The number of primordial germ cells (PGCs), the progenitors of gametes, was significantly increased with both dbcAMP and LIF supplementation. However, supplementation with 0.05 mM dbcAMP and 10 ng/ml LIF combined did not provide any additional effect on this feature. © 2006 Taylor & Francis Group, LLC.