Publication: A novel neuroprotective agent with antioxidant and nitric oxide synthase inhibitory action
Issued Date
2006-07-01
Resource Type
ISSN
10292470
10715762
10715762
Other identifier(s)
2-s2.0-33749333427
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Free Radical Research. Vol.40, No.7 (2006), 685-695
Suggested Citation
Opa Vajragupta, Chantana Boonyarat, Yukihisa Murakami, Michihisa Tohda, Kinzo Musatmoto, Arthur J. Olson, Hiroshi Watanabe A novel neuroprotective agent with antioxidant and nitric oxide synthase inhibitory action. Free Radical Research. Vol.40, No.7 (2006), 685-695. doi:10.1080/10715760500423763 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23012
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
A novel neuroprotective agent with antioxidant and nitric oxide synthase inhibitory action
Other Contributor(s)
Abstract
Nα-vanillyl-Nα-nitroarginine (N - 1) that combines the active functions of natural antioxidant and nitric oxide synthase inhibitor was developed for its neuroprotective properties. N - 1 exhibited protective effects against hydrogen peroxide-induced cell damage and the inhibitory effect on nitric oxide 'NO' production induced by calcium ionophore in NG 108-15 cells. N - 1 inhibited the constitutive NOS isolated from rat cerebellar in a greater extent than constitutive NOS from human endothelial cells. Low binding energy (-10.2 kcal/mol) obtained from docking N - 1 to nNOS supported the additional mode of action of N - 1 as an nNOS inhibitor. The in vivo neuroprotective effect on kainic acid-induced nitric oxide production and neuronal cell death in rat brain was investigated via microdialysis. Rats were injected intra-peritonially with N - 1 at 75 μmol/kg before kainic acid injection (10 mg/kg). The significant suppression effect on kainic acid-induced NO and significant increase in surviving cells were observed in the hippocampus at 40 min after the induction.