Publication: Unstable lysogeny and pseudolysogeny in Vibrio harveyi siphovirus-like phage 1
Issued Date
2006-02-01
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ISSN
00992240
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2-s2.0-33144473092
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Mahidol University
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SCOPUS
Bibliographic Citation
Applied and Environmental Microbiology. Vol.72, No.2 (2006), 1355-1363
Suggested Citation
Krit Khemayan, Tirasak Pasharawipas, Orapim Puiprom, Siriporn Sriurairatana, Orasa Suthienkul, Timothy W. Flegel Unstable lysogeny and pseudolysogeny in Vibrio harveyi siphovirus-like phage 1. Applied and Environmental Microbiology. Vol.72, No.2 (2006), 1355-1363. doi:10.1128/AEM.72.2.1355-1363.2006 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23084
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Title
Unstable lysogeny and pseudolysogeny in Vibrio harveyi siphovirus-like phage 1
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Abstract
Exposure of Vibrio harveyi (strain VH1114) to V. harveyi siphovirus-like phage 1 (VHS1) resulted in the production of a low percentage of lysogenized clones of variable stability. These were retrieved most easily as small colonies within dot plaques. Analysis revealed that VHS1 prophage was most likely carried by VH1114 as an episome rather than integrated into the host chromosome. In the late exponential growth phase, lysogenized VH1114 continuously produced VHS1 but also gave rise to a large number of cured progeny. The absence of phage DNA in the cured progeny was confirmed by the absence of VHS1 DNA in Southern blot and PCR assays. Curiously, these very stable, cured subclones did not show the parental phenotype of clear plaques with VHS1 but instead showed turbid plaques, both in overlaid lawns and in dot plaque assays. This phenotypic difference from the original parental isolate suggested that transient lysogeny by VHS1 had resulted in a stable genetic change in the cured clones. Such clones may be called pseudolysogens (i.e., false lysogens), since they have undergone transient lysogeny and have retained some resistance to full lytic phage development, despite the loss of viable or detectable prophage. Copyright © 2006, American Society for Microbiology. All Rights Reserved.