Publication: Induction of specific immune responses against the Plasmodium vivax liver-stage via in vitro activation by dendritic cells
Issued Date
2006-09-01
Resource Type
ISSN
13835769
Other identifier(s)
2-s2.0-33745887751
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasitology International. Vol.55, No.3 (2006), 187-193
Suggested Citation
Prachya Vichchathorn, Rachaneeporn Jenwithisuk, Surasak Leelaudomlipi, Sumalee Tungpradabkul, Suradej Hongeng, Liwang Cui, Jetsumon Sattabongkot, Rachanee Udomsangpetch Induction of specific immune responses against the Plasmodium vivax liver-stage via in vitro activation by dendritic cells. Parasitology International. Vol.55, No.3 (2006), 187-193. doi:10.1016/j.parint.2006.04.001 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23311
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Title
Induction of specific immune responses against the Plasmodium vivax liver-stage via in vitro activation by dendritic cells
Abstract
Due to chronic morbidity, the risk of increasing drug resistance and the existence of the hypnozoite stage in Plasmodium vivax malaria, there is a need to find out how hosts develop immunity to compromise the malaria parasites. Here we focused on an in vitro model for immunotherapy and vaccine development. Immunosuppressive mechanisms in malaria include inhibition of T cell response and suppression of dendritic cell function. Using in vitro activation of lymphocytes by malaria antigen-pulsed dendritic cells could overcome the limitation of antigen presentation during acute infections. Here we showed that the sporozoite-pulsed dendritic cell could elicit cytotoxicity against liver stage of P. vivax. Analysis using immunophenotypic markers showed maturation of the dendritic cells and stimulation of cytotoxic T cells. Functional assay of the in vitro-activated cytotoxic T cells showed enhancement of specific killing of the P. vivax exoerythrocytic stages within infected hepatocytes. This model may be useful for vaccine development against human malaria. © 2006 Elsevier Ireland Ltd. All rights reserved.