Publication: Vitrification of mouse oocyte using open pulled straws compared with needles
Issued Date
2006-12-01
Resource Type
ISSN
01252208
01252208
01252208
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2-s2.0-33845639694
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol.89, No.12 (2006), 2015-2020
Suggested Citation
Surachai Pornwiroon, Somboon Kunathikom, Orawan Makemaharn, Rattanaphorn Huanaraj Vitrification of mouse oocyte using open pulled straws compared with needles. Journal of the Medical Association of Thailand. Vol.89, No.12 (2006), 2015-2020. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23425
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Title
Vitrification of mouse oocyte using open pulled straws compared with needles
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Abstract
Objective: To compare the survival rate of mouse oocytes and fertilization rate between using open pulled straws (OPS) and needles for vitrification. Material and Method: Meiosis II oocytes from female C57B/6J mice aged 7-8 weeks were collected and allocated to two groups for vitrification by using OPS or needles. Vitrified oocytes were thawed, morphological survival and fertilization rate were examined. Results: There was no obvious difference between the morphological survival rates of vitrified mouse oocytes using OPS and needles (66.7% vs 64.8%). Proportions Difference 1.9% (95%CI -7.1, 10.7). The vitrified oocytes from the needle had significantly higher percentages of fertilization rate than OPS (76.8% vs 62.5%). Proportions Difference -14.3% (95%CI -24.5, -3.6). Conclusion: Vitrification method of mouse oocytes using needles when compared to OPS provides a similar morphological survival rate and higher fertilization rate.