Publication: Agrobacterium tumefaciens fur has important physiological roles in iron and manganese homeostasis, the oxidative stress response, and full virulence
Issued Date
2007-08-01
Resource Type
ISSN
00992240
Other identifier(s)
2-s2.0-34547775059
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Applied and Environmental Microbiology. Vol.73, No.15 (2007), 4760-4768
Suggested Citation
Worawan Kitphati, Patchara Ngok-ngam, Sukanya Suwanmaneerat, Rojana Sukchawalit, Skorn Mongkolsuk Agrobacterium tumefaciens fur has important physiological roles in iron and manganese homeostasis, the oxidative stress response, and full virulence. Applied and Environmental Microbiology. Vol.73, No.15 (2007), 4760-4768. doi:10.1128/AEM.00531-07 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23992
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Agrobacterium tumefaciens fur has important physiological roles in iron and manganese homeostasis, the oxidative stress response, and full virulence
Other Contributor(s)
Abstract
In Agrobacterium tumefaciens, the balance between acquiring enough iron and avoiding iron-induced toxicity is regulated in part by Fur (ferric uptake regulator). A fur mutant was constructed to address the physiological role of the regulator. Atypically, the mutant did not show alterations in the levels of siderophore biosynthesis and the expression of iron transport genes. However, the fur mutant was more sensitive than the wild type to an iron chelator, 2,2′-dipyridyl, and was also more resistant to an iron-activated antibiotic, streptonigrin, suggesting that Fur has a role in regulating iron concentrations. A. tumefaciens sitA, the periplasmic binding protein of a putative ABC-type iron and manganese transport system (sitABCD), was strongly repressed by Mn2+ and, to a lesser extent, by Fe2+, and this regulation was Fur dependent. Moreover, the fur mutant was more sensitive to manganese than the wild type. This was consistent with the fact that the fur mutant showed constitutive up-expression of the manganese uptake sit operon. FurAt showed a regulatory role under iron-limiting conditions. Furthermore, Fur has a role in determining oxidative resistance levels. The fur mutant was hypersensitive to hydrogen peroxide and had reduced catalase activity. The virulence assay showed that the fur mutant had a reduced ability to cause tumors on tobacco leaves compared to wild-type NTL4. Copyright © 2007, American Society for Microbiology. All Rights Reserved.