Publication: Induction of RNA-mediated resistance to papaya ringspot virus type W
Issued Date
2007-05-01
Resource Type
ISSN
02191024
12258687
12258687
Other identifier(s)
2-s2.0-34249694199
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Biochemistry and Molecular Biology. Vol.40, No.3 (2007), 404-411
Suggested Citation
Pongrit Krubphachaya, Míla Juříček, Sunee Kertbundit Induction of RNA-mediated resistance to papaya ringspot virus type W. Journal of Biochemistry and Molecular Biology. Vol.40, No.3 (2007), 404-411. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24201
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Title
Induction of RNA-mediated resistance to papaya ringspot virus type W
Author(s)
Abstract
Transformation of cantaloupes with the coat protein (cp) gene of papaya ringspot virus type W (PRSV-W), Thai isolate, was used to introduce virus resistance. Binary vectors containing either the full length coat protein coding region under control of the 35S CaMV promoter (pSA1175), or the inverted-repeat of a coat protein coding region (pSA1304), were constructed and used for Agrobacterium-mediated transformation of cotyledonary explants of the cantaloupe cultivar Sun Lady. Four independent transgenic lines were obtained using pSA1304 and one using pSA1175. Integration of the PRSV-W cp gene into the genome of these transgenic lines was verified by PCR amplification, GUS assays and Southern blot hybridization. In vitro inoculation of these lines with PRSV-W revealed that whereas the line containing pSA1175 remained sensitive, the four lines containing pSA1304 were resistant. The presence of small RNA species, presumably siRNA, corresponding to regions of the viral cp gene in transgenic lines resistant to PRSV-W supports the involvement of post-transcriptional gene silencing in the establishment of resistance.