Publication: Chitosan scaffolds for in vitro buffalo embryonic stem-like cell culture: An approach to tissue engineering
Issued Date
2007-01-01
Resource Type
ISSN
15524981
00219304
00219304
Other identifier(s)
2-s2.0-33845950936
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Biomedical Materials Research - Part B Applied Biomaterials. Vol.80, No.1 (2007), 92-101
Suggested Citation
Wah W. Thein-Han, Yindee Kitiyanant Chitosan scaffolds for in vitro buffalo embryonic stem-like cell culture: An approach to tissue engineering. Journal of Biomedical Materials Research - Part B Applied Biomaterials. Vol.80, No.1 (2007), 92-101. doi:10.1002/jbm.b.30573 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24453
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Chitosan scaffolds for in vitro buffalo embryonic stem-like cell culture: An approach to tissue engineering
Author(s)
Abstract
Three-dimensional (3D) porous chitosan scaffolds are attractive candidates for tissue engineering applications. Chitosan scaffolds of 70, 88, and 95% degree of deacetylation (% DD) with the same molecular weight were developed and their properties with buffalo embryonic stem-like (ES-like) cells were investigated in vitro. Scaffolds were fabricated by freezing and lyophilization. They showed open pore structure with interconnecting pores under scanning electron microscopy (SEM). Higher % DD chitosan scaffolds had greater mechanical strength, slower degradation rate, lower water uptake ability, but similar water retention ability, when compared to lower % DD chitosan. As a strategy to tissue engineering, buffalo ES-like cells were cultured on scaffolds for 28 days. It appeared that chitosan was cytocompatible and cells proliferated well on 88 and 95% DD scaffolds. In addition, the buffalo ES-like cells maintained their pluripotency during the culture period. Furthermore, the SEM and histological study showed that the polygonal buffalo ES-like cells proliferated well and attached to the pores. This study proved that 3D biodegradable highly deacetylated chitosan scaffolds are promising candidates for ES-like cell based tissue engineering and this chitosan scaffold and ES cell based system can be used as in vitro model for subsequent clinical applications. © 2006 Wiley Periodicals, Inc.