Publication: Effect of stevioside on PAH transport by isolated perfused rabbit renal proximal tubule
Issued Date
2000-01-01
Resource Type
ISSN
00084212
DOI
Other identifier(s)
2-s2.0-0033798146
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Canadian Journal of Physiology and Pharmacology. Vol.78, No.9 (2000), 737-744
Suggested Citation
P. Jutabha, C. Toskulkao, V. Chatsudthipong Effect of stevioside on PAH transport by isolated perfused rabbit renal proximal tubule. Canadian Journal of Physiology and Pharmacology. Vol.78, No.9 (2000), 737-744. doi:10.1139/y00-051 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/25885
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Effect of stevioside on PAH transport by isolated perfused rabbit renal proximal tubule
Author(s)
Other Contributor(s)
Abstract
Stevioside, a non-caloric sweetening agent, is used as a sugar substitute. An influence of stevioside on renal function has been suggested, but little is known about its effect on tubular function. Therefore, the present study was designed to explore the direct effect of stevioside on transepithelial transport of p-aminohippurate (PAH) in isolated S2segments of rabbit proximal renal tubules using in vitro microperfusion. Addition of stevioside at a concentration of 0.45 mM to either the tubular lumen, bathing medium, or both at the same time had no effect on transepithelial transport of PAH. Similarly, a concentration of 0.70 mM (maximum solubility in the buffer) when present in the lumen, had no effect on PAH transport. However, this concentration in the bathing medium inhibited PAH transport significantly by about 25-35%. The inhibitory effect of stevioside was gradually abolished after it was removed from the bath. Addition of 0.70 mM stevioside to both lumen and bathing indium at the same time produced no added inhibitory effect. Stevioside at this concentration has no effect on Na+/K+-ATPase activity as well as cell ATP content. These findings suggest that stevioside, at a pharmacological concentration of 0.70 mM, inhibits transepithelial transport of PAH by interfering with the basolateral entry step, the rate-limiting step for transepithelial transport. The lack of effect of stevioside on transepithelial transport of PAH on the luminal side and its reversible inhibitory effect on the basolateral side indicate that stevioside does not permanently change PAH transport and should not harm renal tubular function at normal human intake levels.