Publication: Aberrant antigenic expression in extranodal NK/T-cell lymphoma: a multi-parameter study from Thailand
Issued Date
2011
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eng
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Mahidol University
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BioMed Central
Bibliographic Citation
Diagnostic Pathology. Vol. 6, (2011), 79
Suggested Citation
Tawatchai Pongpruttipan, Tanawan Kummalue, Anan Bedavanija, Archrob Khuhapinant, Koichi Ohshima, Fumiko Arakawa, Daisuke Niino, Sanya Sukpanichnant Aberrant antigenic expression in extranodal NK/T-cell lymphoma: a multi-parameter study from Thailand. Diagnostic Pathology. Vol. 6, (2011), 79. doi:10.1186/1746-1596-6-79 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/2640
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Title
Aberrant antigenic expression in extranodal NK/T-cell lymphoma: a multi-parameter study from Thailand
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Abstract
Background: Extranodal NK/T-cell lymphoma, nasal type (ENKTL) is not common worldwide, but it is the most
common T- and NK-cell lymphomas in many Asian countries. Immunophenotypic profiles were studied based on
limited series. The authors, therefore, studied on ENKTL according to characterize immunophenotypic profiles as
well as the distribution of EBV subtype and LMP-1 gene deletion.
Methods: By using tissue microarray (TMA), immunohistochemical study and EBV encoded RNA (EBER) in situ
hybridization were performed. T-cell receptor (TCR) gene rearrangement, EBV subtyping, and LMP-1 gene deletion
were studied on the available cases.
Results: There were 22 cases eligible for TMA. ENKTL were positive for CD3 (91%), CD5 (9%), CD7 (32%), CD4
(14%), CD56 (82%), TIA-1 (100%), granzyme B (95%), perforin (86%), CD45 (83%), CD30 (75%), Oct2 (25%), and IRF4/
MUM1 (33%). None of them was positive for bF1, CD8, or CD57. TCR gene rearrangement was negative in all 18
tested cases. EBV was subtype A in all 15 tested cases, with 87% deleted LMP-1 gene. Cases lacking perforin
expression demonstrated a significantly poorer survival outcome (p = 0.008).
Conclusions: The present study demonstrated TIA-1 and EBER as the two most sensitive markers. There were a
few CD3 and/or CD56 negative cases noted. Interestingly, losses of CD45 and/or CD7 were not uncommon while
Oct2 and IRF4/MUM1 could be positive in a subset of cases. Based on the present study in conjunction with the
literature review, determination of PCR-based TCR gene rearrangement analysis might not be a useful technique
for making diagnosis of ENKTL.