Publication: Recombinant Enterobacter amnigenus highly toxic to Anopheles dirus mosquito larvae
Issued Date
2001-12-13
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ISSN
03438651
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2-s2.0-0035193034
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Mahidol University
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SCOPUS
Bibliographic Citation
Current Microbiology. Vol.43, No.6 (2001), 448-451
Suggested Citation
Pawjai Khampang, Plearnpis Luxananil, Sutipa Tanapongpipat, Wipa Chungjatupornchai, Sakol Panyim Recombinant Enterobacter amnigenus highly toxic to Anopheles dirus mosquito larvae. Current Microbiology. Vol.43, No.6 (2001), 448-451. doi:10.1007/s002840010337 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/26547
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Title
Recombinant Enterobacter amnigenus highly toxic to Anopheles dirus mosquito larvae
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Abstract
The mosquito-larvicidal binary toxin of Bacillus sphaericus 2297 was expressed in Enterobacter amnigenus, a Gram-negative bacterium isolated from Anopheles dirus larvae gut. The toxin was placed under the regulation of various promoters in order to improve the expression level of the toxin. Amongst the recombinants obtained, E. amnigenus harboring pBS373, a plasmid which contains the toxin genes under the control of the native B. sphaericus promoter, expressed a significant amount of protein, comparable to that found in B. sphaericus 2297. In addition, this recombinant provided approximately twenty times higher toxicity against second-instar Anopheles dirus larvae when compared to B. sphaericus 2297. The procedure of obtaining this environmentally isolated bacterium from larvae gut and introducing the system for mosquito-larvicidal toxin synthesis is noteworthy. The promising result presented here provides a substantial degree of confidence for further field studies.