Publication: Changes in circulating 25-hydroxyvitamin D according to vitamin D binding protein genotypes after vitamin D3 or D2 supplementation
Issued Date
2013
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eng
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Mahidol University
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BioMed Central
Bibliographic Citation
Nutrition Journal. Vol. 12, (2013), 39
Suggested Citation
Hataikarn Nimitphong, Sunee Saetung, Suwannee Chanprasertyotin, La-or Chailurkit, Boonsong Ongphiphadhanakul Changes in circulating 25-hydroxyvitamin D according to vitamin D binding protein genotypes after vitamin D3 or D2 supplementation. Nutrition Journal. Vol. 12, (2013), 39. doi:10.1186/1475-2891-12-39 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/2699
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Title
Changes in circulating 25-hydroxyvitamin D according to vitamin D binding protein genotypes after vitamin D3 or D2 supplementation
Abstract
Background: It is not known whether genetic variation in the vitamin D binding protein (DBP) influences
25-hydroxyvitamin D levels [25(OH)D] after vitamin D supplementation. We aimed to investigate the changes of
total 25(OH)D, 25(OH)D3 and 25(OH)D2 in a Thai cohort, according to type of vitamin D supplement (vitamin D3 or
D2) and DBP genotype, after receiving vitamin D3 or D2 for 3 months.
Methods: Thirty-nine healthy subjects completed the study. All subjects received 400 IU of either vitamin D3 or D2,
plus a calcium supplement, every day for 3 months. Total serum 25(OH)D, 25(OH)D3 and 25(OH)D2 were measured
by LC-MS/MS. Individual genotyping of rs4588 in the DBP gene was performed using real-time PCR.
Results: Vitamin D3 supplementation of 400 IU/d increased 25(OH)D3 significantly (+16.2 ± 4.2 nmol/L, p <0.001).
Vitamin D2 (400 IU/d) caused increased 25(OH)D2 levels (+22.0 ± 2.11 nmol/L, p <0.001), together with a decrease of
25(OH)D3 (−14.2 ± 2.0 nmol/L, p <0.001). At 3 month, subjects in vitamin D3 group tended to have higher total
25(OH)D levels than those in vitamin D2 (67.8 ± 3.9 vs. 61.0 ± 3.0 nmol/L; p = 0.08). Subjects were then classified into
two subgroups: homozygous for the DBP rs4588 C allele (CC), and the rest (CA or AA). With D3 supplementation,
subjects with CA or AA alleles had significantly less increase in 25(OH)D3 and total 25(OH)D when compared with
those with the CC allele. However, no difference was found when the supplement was vitamin D2.
Conclusion: Genetic variation in DBP (rs4588 SNP) influences responsiveness to vitamin D3 but not vitamin D2.