Publication: Mutation in sco affects cytochrome c assembly and alters oxidative stress resistance in Agrobacterium tumefaciens
Issued Date
2009-04-01
Resource Type
ISSN
15746968
03781097
03781097
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2-s2.0-61449229276
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Mahidol University
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SCOPUS
Bibliographic Citation
FEMS Microbiology Letters. Vol.293, No.1 (2009), 122-129
Suggested Citation
Panatda Saenkham, Paiboon Vattanaviboon, Skorn Mongkolsuk Mutation in sco affects cytochrome c assembly and alters oxidative stress resistance in Agrobacterium tumefaciens. FEMS Microbiology Letters. Vol.293, No.1 (2009), 122-129. doi:10.1111/j.1574-6968.2009.01516.x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27259
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Title
Mutation in sco affects cytochrome c assembly and alters oxidative stress resistance in Agrobacterium tumefaciens
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Abstract
Sco (for the synthesis of cytochrome c oxidase) is a mitochondrial membrane protein essential for the correct assembly of cytochrome c oxidase. sco homolog genes exist in a wide variety of bacterial species. Inactivation of Agrobacterium tumefaciens sco leads to markedly decreased cytochrome c oxidase activity. This phenotype can be complemented by either supplementing the culture medium with copper or by a plasmid containing sco. The sco mutant also alters resistance to a superoxide generator menadione and H2O2. Mutational analysis of conserved cysteine residues and a histidine residue within the putative copper ions binding motif of Sco indicates that these residues are essential for the biological activity of Sco. To summarize, we find that A. tumefaciens sco is required for the delivery of copper into active cytochrome c oxidase and in maintaining optimal resistance levels to oxidative stress. © 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.