Publication: Analysis of ultra low genome conservation in Clostridium difficile
Issued Date
2010-12-20
Resource Type
ISSN
19326203
Other identifier(s)
2-s2.0-78650114015
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
PLoS ONE. Vol.5, No.12 (2010)
Suggested Citation
Joy Scaria, Lalit Ponnala, Tavan Janvilisri, Weiwei Yan, Lukas A. Mueller, Yung Fu Chang Analysis of ultra low genome conservation in Clostridium difficile. PLoS ONE. Vol.5, No.12 (2010). doi:10.1371/journal.pone.0015147 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28418
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Analysis of ultra low genome conservation in Clostridium difficile
Other Contributor(s)
Abstract
Microarray-based comparative genome hybridisations (CGH) and genome sequencing of Clostridium difficile isolates have shown that the genomes of this species are highly variable. To further characterize their genome variation, we employed integration of data from CGH, genome sequencing and putative cellular pathways. Transcontinental strain comparison using CGH data confirmed the emergence of a human-specific hypervirulent cluster. However, there was no correlation between total toxin production and hypervirulent phenotype, indicating the possibility of involvement of additional factors towards hypervirulence. Calculation of C. difficile core and pan genome size using CGH and sequence data estimated that the core genome is composed of 947 to 1,033 genes and a pan genome comprised of 9,640 genes. The reconstruction, annotation and analysis of cellular pathways revealed highly conserved pathways despite large genome variation. However, few pathways such as tetrahydrofolate biosynthesis were found to be variable and could be contributing to adaptation towards virulence such as antibiotic resistance. © 2010 Scaria et al.