Publication: Determination of pheniramine enantiomers in eye drop by capillary electrophoresis using hydroxypropyl-β-cyclodextrin as chiral selector
Issued Date
2010-07-01
Resource Type
ISSN
10619348
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2-s2.0-77955758211
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Analytical Chemistry. Vol.65, No.7 (2010), 755-759
Suggested Citation
K. Phatthiyaphaibun, W. Som-Aum, M. Srisa-Ard, J. Threeprom Determination of pheniramine enantiomers in eye drop by capillary electrophoresis using hydroxypropyl-β-cyclodextrin as chiral selector. Journal of Analytical Chemistry. Vol.65, No.7 (2010), 755-759. doi:10.1134/S1061934810070166 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28935
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Title
Determination of pheniramine enantiomers in eye drop by capillary electrophoresis using hydroxypropyl-β-cyclodextrin as chiral selector
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Abstract
A capillary zone electrophoresis procedure has been developed for the chiral determination of pheniramine in eye drop. Native and derivative cyclodextrins (CDs) including γ-CD, β-CD, hydroxypropyl-β-CD and dimethyl-β-CD were tested as chiral selectors. Using 30 mM hydroxypropyl-β-CD in 50 mM phosphate buffer (pH 3.0), the acceptable resolution value (R = 1.55) was obtained. The assay was validated for linearity (3.3 × 10-6-5.0 × 10-4M; R2= 0.9996), limit of detection (3.3 × 10-6M), limit of quantification (8.5 × 10-6M), analytical precision by terms of intra- and inter-day variability (RSD ≤ 2.57%), and accuracy (recovery ≥ 89.3%). The content of pheniramine in eye drop obtained by the proposed method was in good agreement with the declared value. The results indicated that pheniramine in the eye drop was present as the racemate. © 2010 Pleiades Publishing, Ltd.