Publication: Cellulose acetate membrane precipitin (CAP) test in amoebiasis using antigens from four different strains of Entamoeba histolytica
Issued Date
1981-12-01
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ISSN
00383619
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2-s2.0-0019834079
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.12, No.3 (1981), 353-356
Suggested Citation
N. Thammapalerd, S. Tharavanij, P. Samrejrongroj Cellulose acetate membrane precipitin (CAP) test in amoebiasis using antigens from four different strains of Entamoeba histolytica. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.12, No.3 (1981), 353-356. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/30181
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Title
Cellulose acetate membrane precipitin (CAP) test in amoebiasis using antigens from four different strains of Entamoeba histolytica
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Abstract
A simple cellulose acetate membrane precipitin (CAP) test was evaluated against an immunoelectrophoresis (IEP) test using saline extract from 4 different strains (HK-9, HT-10, HT-12 and HT-31) of axenically grown Entamoeba histolytica as the antigens. All 81 sera from patients with amoebic liver abscess were positive in the IEP test against the antigens from all 4 strains. With the CAP test the number positive against antigens from HK-9, HT-10, HT-12 and HT-31 were 79, 77, 79 and 71, respectively. Sera from 100 blood donors were negative by both IEP and CAP tests against antigens from all 4 strains. Comparison between the number of precipitating bands demonstrated by either the IEP or CAP test showed that strain HT-12 was the best source of antigen in exhibiting a significantly greater number of precipitating bands; strain HT-31 was the poorest. The strain HT-10 was comparatively superior to strain HT-31 in the CAP test, whereas in the IEP test strains HK-9 and HT-31 were both inferior to strain HT-10.