Publication: Alu hypomethylation in smoke-exposed epithelia and oral squamous carcinoma
Issued Date
2013-01-01
Resource Type
ISSN
2476762X
15137368
15137368
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2-s2.0-84887596945
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Cancer Prevention. Vol.14, No.9 (2013), 5495-5501
Suggested Citation
Charoenchai Puttipanyalears, Keskanya Subbalekha, Apiwat Mutirangura, Nakarin Kitkumthorn Alu hypomethylation in smoke-exposed epithelia and oral squamous carcinoma. Asian Pacific Journal of Cancer Prevention. Vol.14, No.9 (2013), 5495-5501. doi:10.7314/APJCP.2013.14.9.5495 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31397
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Title
Alu hypomethylation in smoke-exposed epithelia and oral squamous carcinoma
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Abstract
Background: Alu elements are one of the most common repetitive sequences that now constitute more than 10% of the human genome and potential targets for epigenetic alterations. Correspondingly, methylation of these elements can result in a genome-wide event that may have an impact in cancer. However, studies investigating the genome-wide status of Alu methylation in cancer remain limited. Objectives: Oral squamous cell carcinoma (OSCC) presents with high incidence in South-East Asia and thus the aim of this study was to evaluate the Alu methylation status in OSCCs and explore with the possibility of using this information for diagnostic screening. We evaluated Alu methylation status in a) normal oral mucosa compared to OSCC; b) peripheral blood mononuclear cells (PBMCs) of normal controls comparing to oral cancer patients; c) among oral epithelium of normal controls, smokers and oral cancer patients. Materials and Methods: Alu methylation was detected by combined bisulfite restriction analysis (COBRA) at 2 CpG sites. The amplified products were classified into three patterns; hypermethylation (mCmC), partial methylation (uCmC+mCuC), and hypomethylation (uCuC). Results: The results demonstrate that the %mCmC value is suitable for differentiating normal and cancer in oral tissues (p=0.0002), but is not significantly observe in PBMCs. In addition, a stepwise decrease in this value was observed in the oral epithelium from normal, light smoker, heavy smoker, low stage and high stage OSCC (p=0.0003). Furthermore, receiver operating characteristic (ROC) curve analyses demonstrated the potential of combined %mC or %mCmC values as markers for oral cancer detection with sensitivity and specificity of 86.7% and 56.7%, respectively. Conclusions: Alu hypomethylation is likely to be associated with multistep oral carcinogenesis, and might be developed as a screening tool for oral cancer detection.