Publication: Fluorescent chitosan functionalized magnetic polymeric nanoparticles: Cytotoxicity and in vitro evaluation of cellular uptake
Issued Date
2014-01-01
Resource Type
ISSN
15308022
08853282
08853282
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2-s2.0-84908878845
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Biomaterials Applications. Vol.29, No.5 (2014), 761-768
Suggested Citation
Chariya Kaewsaneha, Kulachart Jangpatarapongsa, Tienrat Tangchaikeeree, Duangporn Polpanich, Pramuan Tangboriboonrat Fluorescent chitosan functionalized magnetic polymeric nanoparticles: Cytotoxicity and in vitro evaluation of cellular uptake. Journal of Biomaterials Applications. Vol.29, No.5 (2014), 761-768. doi:10.1177/0885328214540349 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33895
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Title
Fluorescent chitosan functionalized magnetic polymeric nanoparticles: Cytotoxicity and in vitro evaluation of cellular uptake
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Abstract
© The Author(s) 2014. Nanoparticles possessing magnetic and fluorescent properties were fabricated by the covalent attachment of fluorescein isothiocyanate onto magnetic polymeric nanoparticles functionalized by chitosan. The synthesized magnetic polymeric nanoparticles-chitosan/fluorescein isothiocyanate were successfully used for labeling the living organ and blood-related cancer cells, i.e., HeLa, Hep G2, and K562 cells. The cytotoxicity test of nanoparticles at various incubation times indicated the high cell viability (>90%) without morphological change. The confocal microscopy revealed that they could pass through cell membrane within 2 h for K562 cells and 3 h for HeLa and Hep G2 cells and then confine inside cytoplasm of all types of tested cells for at least 24 h. Therefore, the synthesized magnetic polymeric nanoparticles-chitosan/fluorescein isothiocyanate would potentially be used as cell tracking in theranostic applications.