Publication:
Evaluation of chimeric DNA vaccines consisting of premembrane and envelope genes of Japanese encephalitis and dengue viruses as a strategy for reducing induction of dengue virus infection-enhancing antibody response

Issued Date

2014-02-01

Resource Type

Article

ISSN

13480421
03855600

DOI

10.1111/1348-0421.12125

Other identifier(s)

2-s2.0-84894029501

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Microbiology and Immunology. Vol.58, No.2 (2014), 126-134

Suggested Citation

Fithriyah Sjatha, Miwa Kuwahara, T. Mirawati Sudiro, Masanori Kameoka, Eiji Konishi Evaluation of chimeric DNA vaccines consisting of premembrane and envelope genes of Japanese encephalitis and dengue viruses as a strategy for reducing induction of dengue virus infection-enhancing antibody response. Microbiology and Immunology. Vol.58, No.2 (2014), 126-134. doi:10.1111/1348-0421.12125 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33993

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Title

Evaluation of chimeric DNA vaccines consisting of premembrane and envelope genes of Japanese encephalitis and dengue viruses as a strategy for reducing induction of dengue virus infection-enhancing antibody response

Author(s)

Fithriyah Sjatha
 Miwa Kuwahara
 T. Mirawati Sudiro
 Masanori Kameoka
 Eiji Konishi

Other Contributor(s)

Kobe University School of Medicine
 Universitas Indonesia
 Mahidol University

Abstract

Neutralizing antibodies induced by dengue virus (DENV) infection show viral infection-enhancing activities at sub-neutralizing doses. On the other hand, preimmunity against Japanese encephalitis virus (JEV), a congener of DENV, does not increase the severity of DENV infection. Several studies have demonstrated that neutralizing epitopes in the genus Flavivirus are mainly located in domain III (DIII) of the envelope (E) protein. In this study, chimeric premembrane and envelope (prM-E) gene-based expression plasmids of JEV and DENV1 with DIII substitution of each virus were constructed for use as DNA vaccines and their immunogenicity evaluated. Sera from C3H/He and ICR mice immunized with a chimeric gene containing DENV1 DIII on a JEV prM-E gene backbone showed high neutralizing antibody titers with less DENV infection-enhancing activity. Our results confirm the applicability of this approach as a new dengue vaccine development strategy. © 2014 The Societies and Wiley Publishing Asia Pty Ltd.

Keyword(s)

Immunology and Microbiology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/33993

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