Publication: Sulfated galactans isolated from the red seaweed Gracilaria fisheri target the envelope proteins of white spot syndrome virus and protect against viral infection in shrimp haemocytes
Issued Date
2014-01-01
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ISSN
14652099
00221317
00221317
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2-s2.0-84899676562
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of General Virology. Vol.95, No.PART 5 (2014), 1126-1134
Suggested Citation
Tawut Rudtanatip, Somluk Asuvapongpatana, Boonsirm Withyachumnarnkul, Kanokpan Wongprasert Sulfated galactans isolated from the red seaweed Gracilaria fisheri target the envelope proteins of white spot syndrome virus and protect against viral infection in shrimp haemocytes. Journal of General Virology. Vol.95, No.PART 5 (2014), 1126-1134. doi:10.1099/vir.0.062919-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/34018
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Title
Sulfated galactans isolated from the red seaweed Gracilaria fisheri target the envelope proteins of white spot syndrome virus and protect against viral infection in shrimp haemocytes
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Abstract
The present study was aimed at evaluating an underlying mechanism of the antiviral activity of the sulfated galactans (SG) isolated from the red seaweed Gracilaria fisheri against white spot syndrome virus (WSSV) infection in haemocytes of the black tiger shrimp Penaeus monodon. Primary culture of haemocytes from Penaeus monodon was performed and inoculated with WSSV, after which the cytopathic effect (CPE), cell viability and viral load were determined. Haemocytes treated with WSSV-SG pre-mix showed decreased CPE, viral load and cell mortality from the viral infection. Solid-phase virus-binding assays revealed that SG bound to WSSV in a dose-related manner. Far Western blotting analysis indicated that SG bound to VP 26 and VP 28 proteins of WSSV. In contrast to the native SG, desulfated SG did not reduce CPE and cell mortality, and showed low binding activity with WSSV. The current study suggests that SG from Gracilaria fisheri elicits its anti-WSSV activity by binding to viral proteins that are important for the process of viral attachment to the host cells. It is anticipated that the sulfate groups of SG are important for viral binding. © 2014 The Authors.