Publication: Enhanced human mesenchymal stem cell survival under oxidative stress by overexpression of secreted frizzled-related protein 2 gene
Issued Date
2015-01-13
Resource Type
ISSN
14320584
09395555
09395555
Other identifier(s)
2-s2.0-84926611902
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Annals of Hematology. Vol.94, No.2 (2015), 319-327
Suggested Citation
Kanjana Pomduk, Pakpoom Kheolamai, Yaowalak U-Pratya, Methichit Wattanapanitch, Nuttha Klincumhom, Surapol Issaragrisil Enhanced human mesenchymal stem cell survival under oxidative stress by overexpression of secreted frizzled-related protein 2 gene. Annals of Hematology. Vol.94, No.2 (2015), 319-327. doi:10.1007/s00277-014-2210-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/36527
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Enhanced human mesenchymal stem cell survival under oxidative stress by overexpression of secreted frizzled-related protein 2 gene
Other Contributor(s)
Abstract
© 2014, Springer-Verlag Berlin Heidelberg. Human mesenchymal stem cells (hMSCs) have been used to improve engraftment and to treat graft versus host disease following allogeneic hematopoietic stem cell transplantation. However, oxidative stress presented in the microenvironment can damage the transplanted hMSCs and therefore reduce their survival in target organs. We investigated how to enhance the survival of hMSCs under oxidative stress by overexpressing secreted frizzled-related protein 2 (sFRP2) gene in bone marrow-derived hMSCs and umbilical cord-derived hMSCs. The survival and characteristics of those sFRP2-overexpressing hMSCs (sFRP2-BM-hMSCs and sFRP2-UC-hMSCs) were studied compared with non-transduced hMSCs. We found that the percentages of viable cells in culture of sFRP2-BM-hMSCs and sFRP2-UC-hMSCs in the absence or presence of 0.75 mM H2O2were significantly higher than those of their non-transduced counterparts. The overexpression of sFRP2 gene did not affect the characteristics of hMSCs regarding their morphology, surface marker expression, and differentiation potential. Our study suggests that overexpression of sFRP2 gene in hMSCs might improve the therapeutic effectiveness of hMSC transplantation.