Publication: Variable number of tandem repeats of 9 Plasmodium vivax genes among Southeast Asian isolates
Issued Date
2017-06-01
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ISSN
18736254
0001706X
0001706X
Other identifier(s)
2-s2.0-85014868895
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Mahidol University
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SCOPUS
Bibliographic Citation
Acta Tropica. Vol.170, (2017), 161-168
Suggested Citation
Bo Wang, Myat Htut Nyunt, Seung Gyu Yun, Feng Lu, Yang Cheng, Jin Hee Han, Kwon Soo Ha, Won Sun Park, Seok Ho Hong, Chae Seung Lim, Jun Cao, Jetsumon Sattabongkot, Myat Phone Kyaw, Liwang Cui, Eun Taek Han Variable number of tandem repeats of 9 Plasmodium vivax genes among Southeast Asian isolates. Acta Tropica. Vol.170, (2017), 161-168. doi:10.1016/j.actatropica.2017.01.013 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41476
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Title
Variable number of tandem repeats of 9 Plasmodium vivax genes among Southeast Asian isolates
Abstract
© 2017 Elsevier B.V. The variable number of tandem repeats (VNTRs) provides valuable information about both the functional and evolutionary aspects of genetic diversity. Comparative analysis of 3 Plasmodium falciparum genomes has shown that more than 9% of its open reading frames (ORFs) harbor VNTRs. Although microsatellites and VNTR genes of P. vivax were reported, the VNTR polymorphism of genes has not been examined widely. In this study, 230 P. vivax genes were analyzed for VNTRs by SERV, and 33 kinds of TR deletions or insertions from 29 P. vivax genes (12.6%) were found. Of these, 9 VNTR fragments from 8 P. vivax genes were used for PCR amplification and sequence analysis to examine the genetic diversity among 134 isolates from four Southeast Asian countries (China, Republic of Korea, Thailand, and Myanmar) with different malaria endemicity. We confirmed the existence of extensive polymorphism of VNTR fragments in field isolates. This detection provides several suitable markers for analysis of the molecular epidemiology of P. vivax field isolates.