Publication: Molecular detection and genetic diversity of bovine Babesia spp., Theileria orientalis, and Anaplasma marginale in beef cattle in Thailand
Issued Date
2017-02-01
Resource Type
ISSN
14321955
09320113
09320113
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2-s2.0-85007471016
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasitology Research. Vol.116, No.2 (2017), 751-762
Suggested Citation
Charoonluk Jirapattharasate, Paul Franck Adjou Moumouni, Shinuo Cao, Aiko Iguchi, Mingming Liu, Guanbo Wang, Mo Zhou, Patrick Vudriko, Artemis Efstratiou, Tanasak Changbunjong, Sivapong Sungpradit, Parntep Ratanakorn, Walasinee Moonarmart, Poonyapat Sedwisai, Thekhawet Weluwanarak, Witsanu Wongsawang, Hiroshi Suzuki, Xuenan Xuan Molecular detection and genetic diversity of bovine Babesia spp., Theileria orientalis, and Anaplasma marginale in beef cattle in Thailand. Parasitology Research. Vol.116, No.2 (2017), 751-762. doi:10.1007/s00436-016-5345-2 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41535
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Title
Molecular detection and genetic diversity of bovine Babesia spp., Theileria orientalis, and Anaplasma marginale in beef cattle in Thailand
Author(s)
Charoonluk Jirapattharasate
Paul Franck Adjou Moumouni
Shinuo Cao
Aiko Iguchi
Mingming Liu
Guanbo Wang
Mo Zhou
Patrick Vudriko
Artemis Efstratiou
Tanasak Changbunjong
Sivapong Sungpradit
Parntep Ratanakorn
Walasinee Moonarmart
Poonyapat Sedwisai
Thekhawet Weluwanarak
Witsanu Wongsawang
Hiroshi Suzuki
Xuenan Xuan
Paul Franck Adjou Moumouni
Shinuo Cao
Aiko Iguchi
Mingming Liu
Guanbo Wang
Mo Zhou
Patrick Vudriko
Artemis Efstratiou
Tanasak Changbunjong
Sivapong Sungpradit
Parntep Ratanakorn
Walasinee Moonarmart
Poonyapat Sedwisai
Thekhawet Weluwanarak
Witsanu Wongsawang
Hiroshi Suzuki
Xuenan Xuan
Other Contributor(s)
Abstract
© 2016, Springer-Verlag Berlin Heidelberg. Babesia spp., Theileria orientalis, and Anaplasma marginale are significant tick-borne pathogens that affect the health and productivity of cattle in tropical and subtropical areas. In this study, we used PCR to detect the presence of Babesia bovis, Babesia bigemina, and T. orientalis in 279 beef cattle from Western Thailand and A. marginale in 608 beef cattle from the north, northeastern, and western regions. The PCRs were performed using species-specific primers based on the B. bovis spherical body protein 2 (BboSBP2), B. bigemina rhoptry-associated protein 1a (BbiRAP-1a), T. orientalis major piroplasm surface protein (ToMPSP), and A. marginale major surface protein 4 (AmMSP4) genes. To determine the genetic diversity of the above parasites, amplicons of B. bovis and B. bigemina ITS1-5.8s rRNA gene-ITS2 regions (B. bovis ITS, B. bigemina ITS), ToMPSP, and AmMSP4 genes were sequenced for phylogenetic analysis. PCR results revealed that the prevalence of B. bovis, B. bigemina, T. orientalis, and A. marginale in the Western region was 11.1, 12.5, 7.8, and 39.1 %, respectively. Coinfections of two or three parasites were observed in 17.9 % of the animals sampled. The study revealed that the prevalence of A. marginale in the western region was higher than in the north and northeastern regions (7 %). Sequence analysis showed the BboSBP2 gene to be more conserved than B. bovis ITS in the different isolates and, similarly, the BbiRAP-1a was more conserved than B. bigemina ITS. In the phylogenetic analysis, T. orientalis MPSP sequences were classified into types 3, 5, and 7 as previously reported. A. marginale MSP4 gene sequences shared high identity and similarity with each other and clustered with isolates from other countries. This study provides information on the prevalence and genetic diversity of tick-borne pathogens in beef cattle and highlights the need for effective strategies to control these pathogens in Thailand.