Publication: Protein Network Analysis and Functional Studies of Calcium Oxalate Crystal-Induced Cytotoxicity in Renal Tubular Epithelial Cells
Issued Date
2018-04-01
Resource Type
ISSN
16159861
16159853
16159853
Other identifier(s)
2-s2.0-85044713146
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Proteomics. Vol.18, No.8 (2018)
Suggested Citation
Paleerath Peerapen, Sakdithep Chaiyarit, Visith Thongboonkerd Protein Network Analysis and Functional Studies of Calcium Oxalate Crystal-Induced Cytotoxicity in Renal Tubular Epithelial Cells. Proteomics. Vol.18, No.8 (2018). doi:10.1002/pmic.201800008 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45193
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Protein Network Analysis and Functional Studies of Calcium Oxalate Crystal-Induced Cytotoxicity in Renal Tubular Epithelial Cells
Other Contributor(s)
Abstract
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Our previous expression study has reported a set of proteins with altered levels in renal tubular cells after exposure to calcium oxalate monohydrate (COM) crystals, which are the main composition of kidney stones. However, their functional significance remained largely unknown. In this study, protein network analysis revealed that the significantly altered proteins induced by COM crystals were involved mainly in three main functional networks, including i) cell proliferation and wound healing; ii) oxidative stress and mitochondrial function; and iii) cellular junction complex and integrity. Cell proliferation and wound healing assays showed that the COM-treated cells had defective proliferation and tissue healing capability, respectively. Oxyblot analysis demonstrated accumulation of the oxidized proteins, whereas intracellular ATP level was significantly increased in the COM-treated cells. Additionally, level of zonula occludens-1 (ZO-1), a tight junction protein, was significantly decreased, consistent with the significant declines in transepithelial resistance (TER) and level of RhoA signaling molecule in the COM-treated cells. These findings indicate significant perturbations in mitochondrial and oxidative stress axis that cause defective cell proliferation, tissue healing capability, junctional protein complex, and cellular integrity of renal tubular epithelial cells exposed to COM crystals that may play important roles in kidney stone pathogenesis.