Publication: Quantitation of paracetamol by liquid chromatography-mass spectrometry in human plasma in support of clinical trial
Issued Date
2018-01-01
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ISSN
20565623
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2-s2.0-85069008024
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Mahidol University
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SCOPUS
Bibliographic Citation
Future Science OA. Vol.4, No.8 (2018)
Suggested Citation
Richard Kin Ting Kam, Michael Ho Ming Chan, Hiu Ting Wong, Aniruddha Ghose, Arjen M. Dondorp, Katherine Plewes, Joel Tarning Quantitation of paracetamol by liquid chromatography-mass spectrometry in human plasma in support of clinical trial. Future Science OA. Vol.4, No.8 (2018). doi:10.4155/fsoa-2018-0039 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45320
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Title
Quantitation of paracetamol by liquid chromatography-mass spectrometry in human plasma in support of clinical trial
Abstract
© 2018 Kin Ting Richard Kam. Aim: Paracetamol is a well-tolerated antipyretic widely used in severe malaria management. The study aimed to develop and validate a rapid LC-MS/MS assay to quantify paracetamol in plasma from patients with severe malaria. Materials & methods: Plasma sample was precipitated by organic solvent containing isotope-labeled paracetamol internal standard. Supernatant was isolated, diluted with water, followed by LC-MS/MS analysis. Results: Plasma samples were extracted and assayed in less than 5.5 min. The assay response was linear (0.125-50 mg/l) with total intra- and interassay imprecision of <1.4%, which were considerably lower than most published reports. Conclusion: We developed, validated and applied a rapid and small volume LC-MS/MS assay with high precision and accuracy for plasma paracetamol quantitation in 989 samples from 62 patients with severe malaria. The simple and high-throughput quality could facilitate assay automation for future clinical studies.