Publication: Glutathione S-transferase (GST) of American Cockroach, Periplaneta americana: Classes, Isoforms, and Allergenicity
Issued Date
2018-12-01
Resource Type
ISSN
20452322
Other identifier(s)
2-s2.0-85040467437
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports. Vol.8, No.1 (2018)
Suggested Citation
Nitat Sookrung, Onrapak Reamtong, Rojana Poolphol, Nitaya Indrawattana, Watee Seesuay, Nawannaporn Saelim, Pongsakorn Tantilipikorn, Chaweewan Bunnag, Wanpen Chaicumpa, Anchalee Tungtrongchitr Glutathione S-transferase (GST) of American Cockroach, Periplaneta americana: Classes, Isoforms, and Allergenicity. Scientific Reports. Vol.8, No.1 (2018). doi:10.1038/s41598-017-18759-z Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/47472
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Glutathione S-transferase (GST) of American Cockroach, Periplaneta americana: Classes, Isoforms, and Allergenicity
Other Contributor(s)
Abstract
© 2017 The Author(s). Insect glutathione S-transferases (GSTs) play important roles in insecticide/drug resistance and stress response. Medically, GSTs of house dust mites (Dermatophagoides pteronyssinus and Blomia tropicalis) and German cockroach (Blattella germanica) are human allergens. In this study, classes, isoforms and B-cell and allergenic epitopes of GST of American cockroach, Periplaneta americana, the predominant species in the tropics and subtropics were investigated for the first time. Enzymatically active native and recombinant P. americana-GSTs bound to IgE in sera of all P. americana allergic patients that were tested. By gel-based proteomics and multiple sequence alignments, the native GST comprises three isoforms of delta and sigma classes. All isoforms interacted with serum IgE of the cockroach allergic subjects. Molecularly, the protein contains six B-cell epitopes; two epitopes located at β1-α1 and β4-α3 regions bound to patients' serum IgE, indicating that they are allergenic. P. americana are ubiquitous and their GST can sensitize humans to allergic diseases; thus, the protein should be included in the allergen array for component resolved diagnosis (CRD) of allergic patients, either by skin prick test or specific IgE determination. The GST is suitable also as a target of environmental allergen detection and quantification for intervention of cockroach sensitization and allergic morbidity.