Publication: Down-regulation of mitochondrial NADH and cytochrome b gene associated with high tumor stages in head and neck squamous cell carcinoma
Issued Date
2019-03-01
Resource Type
ISSN
18791506
00039969
00039969
Other identifier(s)
2-s2.0-85059900769
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Mahidol University
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SCOPUS
Bibliographic Citation
Archives of Oral Biology. Vol.99, (2019), 107-112
Suggested Citation
Pitsapaporn Wangpermtam, Songsak Petmitr, Phaibul Punyarit, Boworn Klongnoi, Sirima Sanguansin Down-regulation of mitochondrial NADH and cytochrome b gene associated with high tumor stages in head and neck squamous cell carcinoma. Archives of Oral Biology. Vol.99, (2019), 107-112. doi:10.1016/j.archoralbio.2019.01.005 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50241
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Title
Down-regulation of mitochondrial NADH and cytochrome b gene associated with high tumor stages in head and neck squamous cell carcinoma
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Abstract
© 2019 Elsevier Ltd Objective: This study aimed to determine mitochondrial mRNA expression levels and the relationships between these expression levels and various adverse clinicopathological characteristics. Methods: The mRNA expression levels of all 12 genes encoded protein, located on the heavy-strand of mitochondrial DNA including cytochrome b, NADH1, NADH2, NADH3, NADH4, NADH4L, NADH5, ATPase6, ATPase8, cytochrome c oxidase subunit 1, cytochrome c oxidase subunit 2, cytochrome c oxidase subunit 3 were analyzed in 30 head and neck squamous cell carcinoma (HNSCC) and the corresponding normal tissues using reverse transcriptase quantitative real time PCR. Pearson Chi-square test was used to determine the relationships between these expression levels and categorical parameters. Results: The expression levels of 12 mitochondrial mRNAs were observed in all 30 HNSCC patients with down-regulation, ranging from 43.3% to 76.7% and up-regulation, ranging from 10.0% to 36.7%. Furthermore, the number of cases with down-regulations in all 6 NADH and cytochrome b mRNA with TMN stages III and IV were significantly higher than that in stages I and II (p=0.049 and 0.007, respectively). Conclusion: Down-regulation of all mitochondrial NADH mRNA as well as mitochondrial cytochrome b mRNA was associated with high tumor stage among HNSCC patients.