Publication: Monoclonal antibody-based capture ELISA in the diagnosis of previous dengue infection
Issued Date
2019-10-29
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1743422X
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2-s2.0-85074331461
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Mahidol University
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SCOPUS
Bibliographic Citation
Virology Journal. Vol.16, No.1 (2019)
Suggested Citation
Chukiat Sirivichayakul, Kriengsak Limkittikul, Pornthep Chanthavanich, Sutee Yoksan, Anuttarasakdi Ratchatatat, Jacqueline Kyungah Lim, Watcharee Arunsodsai, Arunee Sabchareon Monoclonal antibody-based capture ELISA in the diagnosis of previous dengue infection. Virology Journal. Vol.16, No.1 (2019). doi:10.1186/s12985-019-1222-9 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/51001
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Title
Monoclonal antibody-based capture ELISA in the diagnosis of previous dengue infection
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Abstract
© 2019 The Author(s). Background: Dengue is an important mosquito-borne disease. There is currently only one licensed vaccine for dengue prevention. The vaccine provides higher efficacy in pre-vaccination dengue-seropositive persons but a higher risk of subsequent more severe dengue in dengue-seronegative persons. It is recommended that the dengue vaccine may be given in dengue-seropositive individuals or as mass vaccination without individual pre-vaccination screening in areas where the dengue seroprevalence is > 80% in children aged 9 years. We evaluated a dengue specific immunoglobulin G monoclonal antibody-based capture enzyme-linked immunosorbent assay (MAb-ELISA) in the diagnosis of previous dengue infection using serum samples from the cohort study in Ratchaburi Province, Thailand. Methods: The MAb-ELISA was compared to 70% plaque reduction neutralization test (PRNT70) in 453 serum samples from children aged 3-11 years in Ratchaburi Province, Thailand. Results: The sensitivity and specificity of MAb-ELISA at the positive to negative (P/N) ratio cut-off level of > 3 were both 0.91 in the diagnosis of previous dengue infection, compared to PRNT70. The false positivity was mainly in Japanese encephalitis (JE) seropositive subjects. Conclusions: This research provides evidence that MAb-ELISA is useful for dengue seroprevalence study and dengue pre-vaccination screening. JE seropositivity was the major cause of false positive result in the study population.