Validation of plasma membrane proteins expressed in invading hepatocellular carcinoma
Issued Date
2014
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Patamaporn Molee, ปัทมาภรณ์ โมลี, Poom Adisakwattana, ภูมิ อดิศักดิ์วัฒนา, Onrapak Reamtong, อรภัค เลี่ยมทอง, Songsak Petmitr, ทรงศักดิ์ เพ็ชรมิตร, Sanya Sukpanichnant, Urai Chaisri, อุไร ไชยศรี (2014). Validation of plasma membrane proteins expressed in invading hepatocellular carcinoma. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/63180
Title
Validation of plasma membrane proteins expressed in invading hepatocellular carcinoma
Other Contributor(s)
Abstract
Introduction: The current gold standards and most commonly available tests for
hepatocellular carcinomar surveillance are assay for serum α-fetoprotein (AFP) and
hepatic ultrasonography. Although widely used, AFP level has limited sensitivity and
specificity for HCC. Newer methods such as biomarkers or radiological assays for early
detection of HCC are urgently needed. This study identified and validated the potential
membrane protein related to invading HCC, which may be of interest for discovering a novel
potential membrane protein contributing to human HCC.
Objective: To identify and validate plasma membrane proteins expressed in invading
HCC
Methods: Cell surface proteins of invading HepG2 were isolated and separated by SDSPAGE.
Protein bands were cut to be analyzed by mass spectrometry. Analysis by mass
spectrometry of proteins was checked by RT-PCR and real time PCR, Western blot and
immunolocalization in cell line, and validated in various grades of tumor tissues by
immunohistochemistry. Correlations of these finding were investigated to gain insights
into potential candidate proteins contributing to invasion and metastasis in human
HCC.
Results: Protein spot named P2 and P3 were identified by mass spectrometry and
validated by real time PCR, western blot and immunolocalization and showed in
various grades of HCC tissues.
Conclusion: 2 and P3 are membrane proteins expressed in invading HCC cell line and
HCC tissues that might be potential proteins contributing to invasion and metastasis
in human HCC.
Description
Joint International Tropical Medicine Meeting 2014: 3D perspectives on tropical medicine: drivers, diversity and determination the 8th seminar on food-and water-borne parasitic zoonoses: 2-4 December 2014: Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2014. p. 260.