Multi-immunodot for rapid differential diagnosis of eosinophilic meningitis due to parasitic infections
Issued Date
2004
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Prapathip Eamsobphana, Jerawan Ongrotchanakun, Adisak Yoolex, Nanthiya Monkong, Phaibulaya Punthuprapasa, Paron Dekumyoy, พารณ ดีคำย้อย (2004). Multi-immunodot for rapid differential diagnosis of eosinophilic meningitis due to parasitic infections. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/63399
Title
Multi-immunodot for rapid differential diagnosis of eosinophilic meningitis due to parasitic infections
Other Contributor(s)
Abstract
A multi-dot enzyme-linked immunosorbent assay (ELISA) was developed for the rapid and simple differential diagnosis of eosinophilic meningitis due to helminth infections. Ultrafiltered, purified antigens of Parastrongylus (=Angiostrongylus) cantonensis, Gnathostoma spinigerum and Taenia solium metacestodes, the most common parasites that invade the central nervous system and cause eosinophilic pleocytosis, were dotted onto a single nitrocellulose membrane strip. Antigen-coated strips, when blocked with 5% skimmed milk and dried, were stable for at least 6 months at 4 degrees C. With peroxidase conjugated anti-human immunoglobulins and 4-chloro-1-naphthol as a substrate, antibodies in the corresponding patients' sera were clearly detected on the membrane strip as well-defined blue dots. Although cross-reactions between P. cantonensis and G. spinigerum antigens were observed with the use of partially purified antigens, the darkest dot correlated well with the infecting parasites in all cases. This fast, easy and economical multiple dot-blot ELISA method is useful for the differential diagnosis of eosinophilic meningitis caused by parasitic helminths, as semi-purified antigens can be easily obtained by ultrafiltration and used. Further improvements using highly specific parasite antigens may make this multi-immunodot test more suitable for wide-scale use in field studies and diagnostic laboratories
Description
Joint International Tropical Medicine Meeting 2004: Ambassador Hotel, Thailand 29 November-1 December 2004: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2004. p.199.