Publication:
Activation of nuclear factor kappa B in peripheral blood mononuclear cells from malaria patients

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tm-ar-srivicha-2012.pdf (842.93 KB)

Accepted Date

2012-06-10

Issued Date

2012-06-10

Copyright Date

2012

Resource Type

Research Article

Language

eng

ISSN

1475-2875 (electronic)

Rights

Mahidol University

Rights Holder(s)

BioMed Central

Bibliographic Citation

Punsawad C, Krudsood S, Maneerat Y, Chaisri U, Tangpukdee N, Pongponratn E. et al. Activation of nuclear factor kappa B in peripheral blood mononuclear cells from malaria patients. Malar J. 2012 Jun 10;11:191.

Suggested Citation

Chuchard Punsawad, ชูชาติ พันธ์สวัสดิ์, Srivicha Krudsood, ศรีวิชา ครุฑสูตร, Yaowapa Maneerat, เยาวพา มณีรัตน์, Urai Chaisri, อุไร ไชยศรี, Noppadon Tangpukdee, นพดล ตั้งภักดี, Emsri Pongponratn, เอี่ยมศรี พงศ์พนรัตน์, Kwannan Nantavisai, Rachanee Udomsangpetch, รัชนีย์ อุดมแสงเพ็ชร, Parnpen Viriyavejakul, พรรณเพ็ญ วิริยเวชกุล Activation of nuclear factor kappa B in peripheral blood mononuclear cells from malaria patients. Punsawad C, Krudsood S, Maneerat Y, Chaisri U, Tangpukdee N, Pongponratn E. et al. Activation of nuclear factor kappa B in peripheral blood mononuclear cells from malaria patients. Malar J. 2012 Jun 10;11:191.. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/736

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Title

Activation of nuclear factor kappa B in peripheral blood mononuclear cells from malaria patients

Author(s)

Chuchard Punsawad
 ชูชาติ พันธ์สวัสดิ์
 Srivicha Krudsood
 ศรีวิชา ครุฑสูตร
 Yaowapa Maneerat
 เยาวพา มณีรัตน์
 Urai Chaisri
 อุไร ไชยศรี
 Noppadon Tangpukdee
 นพดล ตั้งภักดี
 Emsri Pongponratn
 เอี่ยมศรี พงศ์พนรัตน์
 Kwannan Nantavisai
 Rachanee Udomsangpetch
 รัชนีย์ อุดมแสงเพ็ชร
 Parnpen Viriyavejakul
 พรรณเพ็ญ วิริยเวชกุล

Corresponding Author(s)

Parnpen Viriyavejakul

Other Contributor(s)

Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology
 Mahidol University. Faculty of Tropical Medicine. Department of Tropical Hygiene
 Mahidol University. Faculty of Tropical Medicine. Department of Clinical Tropical Medicine
 Mahidol University. Faculty of Science. Department of Pathobiology.

Abstract

BACKGROUND: Malaria parasites and their products can activate a specific immune response by stimulating cytokine production in the host's immune cells. Transcription nuclear factor kappa B (NF-κB) is an important regulator for the control of many pro-inflammatory genes, such as interleukin-1 (IL-1) and tumor necrosis factor (TNF). The activation and expression of NF-κB p65 in peripheral blood mononuclear cells (PBMCs) of malaria patients were investigated and correlated with the levels of IL-10 and TNF to study the nature of NF-κB p65 and its linkage to inflammatory cytokines. METHODS: The sample group comprised 33 patients admitted with malaria caused by Plasmodium vivax (n = 11), uncomplicated Plasmodium falciparum (n = 11), and complicated Plasmodium falciparum (n = 11). Peripheral blood was collected at admission and on day 7 for PBMC isolation. Healthy subjects were used as a control group. The expressions of NF-κB p65 in the PBMCs from malaria patients and the plasma levels of IL-10 and TNF were measured by using enzyme-linked immunosorbent assay (ELISA). The immunofluorescence technique was used to determine NF-κB nuclear translocation. RESULTS: At admission, patients with P. vivax and uncomplicated P. falciparum had significantly elevated phospho-NF-κB p65 levels in the PBMCs compared with those of healthy controls. However, patients with complicated P. falciparum malaria had decreased levels of phospho-NF-κB p65. On day 7 post-treatment, significantly increased phospho-NF-κB p65 was found in the PBMCs of patients with complicated P. falciparum, compared with healthy controls. The plasma level of IL-10 was elevated in day 0 in patients with complicated P. falciparum malaria and was found to be negatively correlated with phospho-NF-κB p65 level (rs = -0.630, p = 0.038). However, there was no correlation between phospho-NF-κB p65 expression and TNF level in patients with complicated P. falciparum malaria. CONCLUSIONS: This is the first report demonstrating alterations in NF-κB p65 activity in the PBMCs of malaria patients. The altered lower features of NF-κB p65 in the PBMCs of patients with complicated P. falciparum at admission could be due to a suppressive effect of high IL-10 associated with complicated P. falciparum malaria.

Keyword(s)

Malaria
 Plasmodium falciparum
 Plasmodium vivax
 Nuclear factor kappa B
 Peripheral blood mononuclear cells
 Interleukin-10
 Tumor necrosis factor
 Open Access article

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https://repository.li.mahidol.ac.th/handle/20.500.14594/736

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TM-Article