Publication:
Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand

Issued Date

2021-12-01

Resource Type

Article

ISSN

20452322

DOI

10.1038/s41598-021-92735-6

Other identifier(s)

2-s2.0-85109727112

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Scientific Reports. Vol.11, No.1 (2021)

Suggested Citation

Nonlawat Boonyalai, Chatchadaporn Thamnurak, Piyaporn Sai-ngam, Winita Ta-aksorn, Montri Arsanok, Nichapat Uthaimongkol, Siratchana Sundrakes, Sorayut Chattrakarn, Chaiyaporn Chaisatit, Chantida Praditpol, Watcharintorn Fagnark, Kirakarn Kirativanich, Suwanna Chaorattanakawee, Pattaraporn Vanachayangkul, Paphavee Lertsethtakarn, Panita Gosi, Darunee Utainnam, Wuttikon Rodkvamtook, Worachet Kuntawunginn, Brian A. Vesely, Michele D. Spring, Mark M. Fukuda, Charlotte Lanteri, Douglas Walsh, David L. Saunders, Philip L. Smith, Mariusz Wojnarski, Narongrid Sirisopana, Norman C. Waters, Krisada Jongsakul, Jariyanart Gaywee Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand. Scientific Reports. Vol.11, No.1 (2021). doi:10.1038/s41598-021-92735-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/79230

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Title

Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand

Author(s)

Nonlawat Boonyalai
 Chatchadaporn Thamnurak
 Piyaporn Sai-ngam
 Winita Ta-aksorn
 Montri Arsanok
 Nichapat Uthaimongkol
 Siratchana Sundrakes
 Sorayut Chattrakarn
 Chaiyaporn Chaisatit
 Chantida Praditpol
 Watcharintorn Fagnark
 Kirakarn Kirativanich
 Suwanna Chaorattanakawee
 Pattaraporn Vanachayangkul
 Paphavee Lertsethtakarn
 Panita Gosi
 Darunee Utainnam
 Wuttikon Rodkvamtook
 Worachet Kuntawunginn
 Brian A. Vesely
 Michele D. Spring
 Mark M. Fukuda
 Charlotte Lanteri
 Douglas Walsh
 David L. Saunders
 Philip L. Smith
 Mariusz Wojnarski
 Narongrid Sirisopana
 Norman C. Waters
 Krisada Jongsakul
 Jariyanart Gaywee

Other Contributor(s)

Armed Forces Research Institute of Medical Sciences, Thailand
 Syracuse VA Medical Center
 Walter Reed Army Institute of Research
 Mahidol University
 U.S. Army Research Institute of Infectious Diseases

Abstract

Malaria remains a public health problem in Thailand, especially along its borders where highly mobile populations can contribute to persistent transmission. This study aimed to determine resistant genotypes and phenotypes of 112 Plasmodium falciparum isolates from patients along the Thai-Cambodia border during 2013–2015. The majority of parasites harbored a pfmdr1-Y184F mutation. A single pfmdr1 copy number had CVIET haplotype of amino acids 72–76 of pfcrt and no pfcytb mutations. All isolates had a single pfk13 point mutation (R539T, R539I, or C580Y), and increased % survival in the ring-stage survival assay (except for R539I). Multiple copies of pfpm2 and pfcrt-F145I were detected in 2014 (12.8%) and increased to 30.4% in 2015. Parasites containing either multiple pfpm2 copies with and without pfcrt-F145I or a single pfpm2 copy with pfcrt-F145I exhibited elevated IC90 values of piperaquine. Collectively, the emergence of these resistance patterns in Thailand near Cambodia border mirrored the reports of dihydroartemisinin-piperaquine treatment failures in the adjacent province of Cambodia, Oddar Meanchey, suggesting a migration of parasites across the border. As malaria elimination efforts ramp up in Southeast Asia, host nations militaries and other groups in border regions need to coordinate the proposed interventions.

Keyword(s)

Multidisciplinary

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/79230

Collections

Scopus 2021